| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human ARHGDIA kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
ARHGDIA |
| Gene ID: |
396 |
| Uniprot ID: |
GDIR1_HUMAN |
| Specificity: |
This kit recognizes Human ARHGDI Alpha in samples. No significant cross-reactivity or interference between Human ARHGDI Alpha and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1. |
| Protein Name | Rho Gdp-Dissociation Inhibitor 1Rho Gdi 1Rho-Gdi Alpha |
| Database Links | Reactome: R-HSA-193634Reactome: R-HSA-209563Reactome: R-HSA-8980692Reactome: R-HSA-9013106Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013407Reactome: R-HSA-9013408 |
| Cellular Localisation | Cytoplasm |
| Alternative CLIA Names | Rho Gdp-Dissociation Inhibitor 1 CLIA kitRho Gdi 1 CLIA kitRho-Gdi Alpha CLIA kitARHGDIA CLIA kitGDIA1 CLIA kit |
| Specificity | This kit recognizes Human ARHGDIα in samples. No significant cross-reactivity or interference between Human ARHGDIα and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 100-116 | 108 |
| EDTA plasma(n=8) | 97-113 | 104 |
| Cell culture media(n=8) | 86-98 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 105.47 | 293.06 | 765.78 | 108.88 | 279.17 | 800.98 |
| Standard deviation | 8.87 | 26.05 | 64.78 | 13.41 | 31.74 | 80.74 |
| CV (%) | 8.41 | 8.89 | 8.46 | 12.32 | 11.37 | 10.08 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
