| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88pg/mL |
| Detection Limit: |
78.13~5000pg/mL |
| Short Description: |
This human APOM kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
APOM |
| Gene ID: |
55937 |
| Uniprot ID: |
APOM_HUMAN |
| Specificity: |
This kit recognizes Human ApoM in samples. No significant cross-reactivity or interference between Human ApoM and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Plasma protein. Expressed in liver and kidney. |
| Function | Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid. |
| Protein Name | Apolipoprotein MApo-MApomProtein G3a |
| Database Links | Reactome: R-HSA-975634 |
| Cellular Localisation | SecretedPresent In High Density Lipoprotein (Hdl) And To A Lesser Extent In Triglyceride-Rich Lipoproteins (Tgrlp) And Low Density Lipoproteins (Ldl) |
| Alternative CLIA Names | Apolipoprotein M CLIA kitApo-M CLIA kitApom CLIA kitProtein G3a CLIA kitAPOM CLIA kitG3A CLIA kitNG20 CLIA kitHSPC336 CLIA kit |
| Specificity | This kit recognizes Human ApoM in samples. No significant cross-reactivity or interference between Human ApoM and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 99-114 | 104 |
| EDTA plasma(n=8) | 90-105 | 97 |
| Cell culture media(n=8) | 98-111 | 105 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 229.43 | 473.10 | 2441.35 | 237.91 | 509.20 | 2261.90 |
| Standard deviation | 28.01 | 38.60 | 216.55 | 24.20 | 41.75 | 232.07 |
| CV (%) | 12.21 | 8.16 | 8.87 | 10.17 | 8.20 | 10.26 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
