Human APOF (Apolipoprotein F) Sandwich ELISA (STJE0004052)

SKU:
STJE0004052

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.47ng/mL
Detection Limit: 0.78~50ng/mL
Short Description: This APOF Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: APOF
Gene ID: 319
Uniprot ID: APOF_HUMAN
Specificity: This kit recognizes Human ApoF in samples. No significant cross-reactivity or interference between Human ApoF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications O-glycosylated with core 1 or possibly core 8 glycans.
Function Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.
Protein Name Apolipoprotein F
Apo-F
Lipid Transfer Inhibitor Protein
Ltip
Database Links Reactome: R-HSA-8964041
Cellular Localisation Secreted
Alternative ELISA Names Apolipoprotein F ELISA kit
Apo-F ELISA kit
Lipid Transfer Inhibitor Protein ELISA kit
Ltip ELISA kit
APOF ELISA kit
Specificity This kit recognizes Human ApoF in samples. No significant cross-reactivity or interference between Human ApoF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 82-93 90
EDTA plasma (n=8) 94-103 100
Cell culture media (n=8) 91-101 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 2.17 5.23 23.43 2.42 5.05 22.81
Standard deviation 0.12 0.26 1.25 0.13 0.25 1.02
CV (%) 5.53 4.97 5.34 5.37 4.95 4.47
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance