• Human APOC2 (Apolipoprotein C-II) Sandwich ELISA (STJE0002247)

Human APOC2 (Apolipoprotein C-II) Sandwich ELISA (STJE0002247)

SKU:
STJE0002247

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 1.88ng/mL
Detection Limit: 3.13~200ng/mL
Short Description: This APOC2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: APOC2
Gene ID: 344
Uniprot ID: APOC2_HUMAN
Specificity: This kit recognizes Human ApoC2 in samples. No significant cross-reactivity or interference between Human ApoC2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Proapolipoprotein C-II is synthesized as a sialic acid containing glycoprotein which is subsequently desialylated prior to its proteolytic processing. Proapolipoprotein C-II, the major form found in plasma undergoes proteolytic cleavage of its N-terminal hexapeptide to generate apolipoprotein C-II, which occurs as the minor form in plasma.
Function Component of chylomicrons, very low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) in plasma. Plays an important role in lipoprotein metabolism as an activator of lipoprotein lipase. Both proapolipoprotein C-II and apolipoprotein C-II can activate lipoprotein lipase. In normolipidemic individuals, it is mainly distributed in the HDL, whereas in hypertriglyceridemic individuals, predominantly found in the VLDL and LDL.
Protein Name Apolipoprotein C-Ii
Apo-Cii
Apoc-Ii
Apolipoprotein C2 Cleaved Into - Proapolipoprotein C-Ii
Proapoc-Ii
Database Links Reactome: R-HSA-8963888
Reactome: R-HSA-8963889
Reactome: R-HSA-8963901
Reactome: R-HSA-8964058
Reactome: R-HSA-9029569
Reactome: R-HSA-975634
Cellular Localisation Secreted
Alternative ELISA Names Apolipoprotein C-Ii ELISA kit
Apo-Cii ELISA kit
Apoc-Ii ELISA kit
Apolipoprotein C2 Cleaved Into - Proapolipoprotein C-Ii ELISA kit
Proapoc-Ii ELISA kit
APOC2 ELISA kit
APC2 ELISA kit
Specificity This kit recognizes Human ApoC2 in samples. No significant cross-reactivity or interference between Human ApoC2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-97 90
EDTA plasma (n=8) 90-102 97
Cell culture media (n=8) 86-96 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 11.00 19.50 83.60 12.10 19.90 86.30
Standard deviation 0.80 0.80 3.90 0.80 0.90 2.90
CV (%) 7.27 4.10 4.67 6.61 4.52 3.36
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance