Human APOA5 (Apolipoprotein A-V) Sandwich ELISA (STJE0004053)

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STJE0004053

£194.50 - £368.50
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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This APOA5 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: APOA5
Gene ID: 116519
Uniprot ID: APOA5_HUMAN
Specificity: This kit recognizes Human ApoA5 in samples. No significant cross-reactivity or interference between Human ApoA5 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Phosphorylated by FAM20C in the extracellular medium.
Function Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and an inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Binds heparin.
Protein Name Apolipoprotein A-V
Apo-Av
Apoa-V
Apolipoprotein A5
Regeneration-Associated Protein 3
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Reactome: R-HSA-8963889
Reactome: R-HSA-8963901
Cellular Localisation Secreted
Early Endosome
Late Endosome
Golgi Apparatus
Trans-Golgi Network
In The Presence Of Sorl1
Internalized To Early Endosomes
Sorted In A Retrograde Fashion To Late Endosomes
From Which A Portion Is Sent To Lysosomes And Degradation
Another Portion Is Sorted To The Trans-Golgi Network
Alternative ELISA Names Apolipoprotein A-V ELISA kit
Apo-Av ELISA kit
Apoa-V ELISA kit
Apolipoprotein A5 ELISA kit
Regeneration-Associated Protein 3 ELISA kit
APOA5 ELISA kit
RAP3 ELISA kit
UNQ411 ELISA kit
PRO773 ELISA kit
Specificity This kit recognizes Human ApoA5 in samples. No significant cross-reactivity or interference between Human ApoA5 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-103 96
EDTA plasma (n=8) 89-100 94
Cell culture media (n=8) 100-109 103

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 168.97 447.94 1846.03 177.36 498.83 1844.50
Standard deviation 10.14 22.40 82.33 9.08 22.60 95.55
CV (%) 6.00 5.00 4.46 5.12 4.53 5.18
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance