| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.56ng/mL |
| Detection Limit: |
0.94~60ng/mL |
| Short Description: |
This human APOA4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
APOA4 |
| Gene ID: |
337 |
| Uniprot ID: |
APOA4_HUMAN |
| Specificity: |
This kit recognizes Human ApoA4 in samples. No significant cross-reactivity or interference between Human ApoA4 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Synthesized primarily in the intestine and secreted in plasma. |
| Post Translational Modifications | Phosphorylation sites are present in the extracellular medium. |
| Function | May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II.potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. |
| Protein Name | Apolipoprotein A-IvApo-AivApoa-IvApolipoprotein A4 |
| Database Links | Reactome: R-HSA-8963888Reactome: R-HSA-8963889Reactome: R-HSA-8963901Reactome: R-HSA-975634Reactome: R-HSA-977225 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Apolipoprotein A-Iv CLIA kitApo-Aiv CLIA kitApoa-Iv CLIA kitApolipoprotein A4 CLIA kitAPOA4 CLIA kit |
| Specificity | This kit recognizes Human ApoA4 in samples. No significant cross-reactivity or interference between Human ApoA4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-103 | 97 |
| EDTA plasma(n=8) | 85-101 | 92 |
| Cell culture media(n=8) | 87-101 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 3.00 | 7.19 | 22.99 | 3.13 | 7.63 | 22.80 |
| Standard deviation | 0.35 | 0.52 | 1.54 | 0.37 | 0.58 | 2.22 |
| CV (%) | 11.67 | 7.23 | 6.70 | 11.82 | 7.60 | 9.74 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
