Human ANG (Angiostatin) Sandwich ELISA (STJE0019699)

SKU:
STJE0019699

Shipping:
Free Shipping
Current Stock:
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25-2000pg/mL
Short Description: This ANG Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: ANG
Gene ID: 283
Uniprot ID: ANGI_HUMAN
Specificity: This kit recognizes Human ANG in samples. No significant cross-reactivity or interference between Human ANG and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Ribonuclease that cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Binds to actin on the surface of endothelial cells.once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.
Protein Name Angiogenin
Ribonuclease 5
Rnase 5
Database Links Reactome: R-HSA-418990
Reactome: R-HSA-9708296
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle Lumen
Secreted
Nucleus
Nucleolus
Rapidly Endocytosed By Target Cells And Translocated To The Nucleus Where It Accumulates In The Nucleolus And Binds To Dna
Alternative ELISA Names Angiogenin ELISA kit
Ribonuclease 5 ELISA kit
Rnase 5 ELISA kit
ANG ELISA kit
RNASE5 ELISA kit
Specificity This kit recognizes Human ANG in samples. No significant cross-reactivity or interference between Human ANG and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-107 97
EDTA plasma (n=8) 94-105 100
Cell culture media (n=8) 94-110 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 97.29 304.43 989.12 101.99 314.65 983.65
Standard deviation 6.22 15.13 33.33 6.21 13.37 34.33
CV (%) 6.39 4.97 3.37 6.09 4.25 3.49
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance