• Western blot analysis of extracts of various cell lines, using FPGS antibody (STJ11104141) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using KCTD9 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

FPGS protein (Recombinant) (STJ11104141)

SKU:
STJ11104141

£146.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-FPGS-protein was developed from rabbit. For use in research applications.
Clonality: Monoclonal
Clone ID: S4141RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FPGS
Gene ID: 2356
Uniprot ID: FOLC_HUMAN
Immunogen Region: 450-587
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 450-587 of human FPGS (Q05932).
Immunogen Sequence: NADQQNFTVTLDQVLLRCLE HQQHWNHLDEEQASPDLWSA PSPEPGGSASLLLAPHPPHT CSASSLVFSCISHALQWISQ GRDPIFQPPSPPKGLLTHPV AHSGASILREAAAIHVLVTG SLHLVGGVLKLLEPALSQ
Function Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Unsubstituted reduced folates are the preferred substrates. Metabolizes methotrexate (MTX) to polyglutamates.
Protein Name Folylpolyglutamate Synthase - Mitochondrial
Folylpoly-Gamma-Glutamate Synthetase
Fpgs
Tetrahydrofolylpolyglutamate Synthase
Tetrahydrofolate Synthase
Database Links Reactome: R-HSA-196757
Cellular Localisation Isoform 1: Mitochondrion Inner Membrane
Mitochondrion Matrix
Isoform 2: Cytoplasm
Alternative Protein Names Folylpolyglutamate Synthase - Mitochondrial protein
Folylpoly-Gamma-Glutamate Synthetase protein
Fpgs protein
Tetrahydrofolylpolyglutamate Synthase protein
Tetrahydrofolate Synthase protein
FPGS protein

Information sourced from Uniprot.org

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