• Immunofluorescence analysis of C6 cells using ZEB1 Rabbit polyclonal antibody (STJ28276) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of ZEB1 in paraffin-embedded mouse testis using ZEB1 Rabbit polyclonal antibody (STJ28276) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ZEB1 in paraffin-embedded rat testis using ZEB1 Rabbit polyclonal antibody (STJ28276) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ZEB1 in paraffin-embedded human mammary cancer using ZEB1 Rabbit polyclonal antibody (STJ28276) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ZEB1 antibody (800-900) (STJ28276)

SKU:
STJ28276

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZEB1 (800-900) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZEB1
Gene ID: 6935
Uniprot ID: ZEB1_HUMAN
Immunogen Region: 800-900
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 800-900 of human ZEB1 (NP_110378.3).
Immunogen Sequence: INIAIPTVTAQLPTIVAIAD QNSVPCLRALAANKQTILIP QVAYTYSTTVSPAVQEPPLK VIQPNGNQDERQDTSSEGVS NVEDQNDSDSTPPKKKMRKT E
Tissue Specificity Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas.
Function Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). In the absence of TGFB1, acts as a repressor of COL1A2 transcription via binding to the E-box in the upstream enhancer region.
Protein Name Zinc Finger E-Box-Binding Homeobox 1
Nil-2-A Zinc Finger Protein
Negative Regulator Of Il2
Transcription Factor 8
Tcf-8
Database Links Reactome: R-HSA-6785807
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Zinc Finger E-Box-Binding Homeobox 1 antibody
Anti-Nil-2-A Zinc Finger Protein antibody
Anti-Negative Regulator Of Il2 antibody
Anti-Transcription Factor 8 antibody
Anti-Tcf-8 antibody
Anti-ZEB1 antibody
Anti-AREB6 antibody
Anti-TCF8 antibody

Information sourced from Uniprot.org

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