• Immunohistochemistry analysis of 14-3-3 gamma in paraffin-embedded Rat spleen using 14-3-3 gamma Rabbit polyclonal antibody (STJ26143) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of 14-3-3 gamma in paraffin-embedded Human colon using 14-3-3 gamma Rabbit polyclonal antibody (STJ26143) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using 14-3-3 gamma Rabbit polyclonal antibody (STJ26143) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 1s.

Anti-YWHAG antibody (100-200) (STJ26143)

SKU:
STJ26143

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-YWHAG (100-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: YWHAG
Gene ID: 7532
Uniprot ID: 1433G_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human 14-3-3 gamma (NP_036611.2).
Immunogen Sequence: VLSLLDNYLIKNCSETQYES KVFYLKMKGDYYRYLAEVAT GEKRATVVESSEKAYSEAHE ISKEHMQPTHPIRLGLALNY SVFYYEIQNAPEQACHLAKT A
Tissue Specificity Highly expressed in brain, skeletal muscle, and heart.
Post Translational Modifications Phosphorylated by various PKC isozymes.
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes inactivation of WDR24 component of the GATOR2 complex by binding to phosphorylated WDR24.
Protein Name 14-3-3 Protein Gamma
Protein Kinase C Inhibitor Protein 1
Kcip-1 Cleaved Into - 14-3-3 Protein Gamma - N-Terminally Processed
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-1445148
Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-5625740
Reactome: R-HSA-5628897
Reactome: R-HSA-75035
Reactome: R-HSA-8854518
Reactome: R-HSA-9614399
Reactome: R-HSA-9735871
Reactome: R-HSA-9755779
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-14-3-3 Protein Gamma antibody
Anti-Protein Kinase C Inhibitor Protein 1 antibody
Anti-Kcip-1 Cleaved Into - 14-3-3 Protein Gamma - N-Terminally Processed antibody
Anti-YWHAG antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance