| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-KU70 (408-609) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
XRCC6 |
| Gene ID: |
2547 |
| Uniprot ID: |
XRCC6_HUMAN |
| Immunogen Region: |
408-609 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 408-609 of human Ku70 (NP_001460.1). |
| Immunogen Sequence: |
PYFVALVPQEEELDDQKIQV TPPGFQLVFLPFADDKRKMP FTEKIMATPEQVGKMKAIVE KLRFTYRSDSFENPVLQQHF RNLEALALDLMEPEQAVDLT LPKVEAMNKRLGSLVDEFKE LVYPPDYNPEGKVTKRKHDN EGSGSKRPKVEYSEEELKTH ISKGTLGKFTVPMLKEACRA YGLKSGLKKQELLEALTKHF QD |
| Post Translational Modifications | Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair. ADP-ribosylated by PARP3. |
| Function | Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA. Required for double-strand break repair and V(D)J recombination. Also has a role in chromosome translocation. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection. Binding to DNA may be mediated by XRCC6. The XRCC5-XRRC6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription. In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway. |
| Protein Name | X-Ray Repair Cross-Complementing Protein 65'-Deoxyribose-5-Phosphate Lyase Ku705'-Drp Lyase Ku7070 Kda Subunit Of Ku AntigenAtp-Dependent Dna Helicase 2 Subunit 1Atp-Dependent Dna Helicase Ii 70 Kda SubunitCtc Box-Binding Factor 75 Kda SubunitCtc75CtcbfDna Repair Protein Xrcc6Lupus Ku Autoantigen Protein P70Ku70Thyroid-Lupus AutoantigenTlaaX-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 6 |
| Database Links | Reactome: R-HSA-164843Reactome: R-HSA-1834949Reactome: R-HSA-3270619Reactome: R-HSA-5693571Reactome: R-HSA-6798695 |
| Cellular Localisation | NucleusChromosome |
| Alternative Antibody Names | Anti-X-Ray Repair Cross-Complementing Protein 6 antibodyAnti-5'-Deoxyribose-5-Phosphate Lyase Ku70 antibodyAnti-5'-Drp Lyase Ku70 antibodyAnti-70 Kda Subunit Of Ku Antigen antibodyAnti-Atp-Dependent Dna Helicase 2 Subunit 1 antibodyAnti-Atp-Dependent Dna Helicase Ii 70 Kda Subunit antibodyAnti-Ctc Box-Binding Factor 75 Kda Subunit antibodyAnti-Ctc75 antibodyAnti-Ctcbf antibodyAnti-Dna Repair Protein Xrcc6 antibodyAnti-Lupus Ku Autoantigen Protein P70 antibodyAnti-Ku70 antibodyAnti-Thyroid-Lupus Autoantigen antibodyAnti-Tlaa antibodyAnti-X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 6 antibodyAnti-XRCC6 antibodyAnti-G22P1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Immunofluorescence analysis of U-2 OS cells using [KD Validated] Ku70 Rabbit polyclonal antibody (STJ11105550) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/178194/370448/STJ11105550_1__75503.1713136400.jpg?c=1 "Immunofluorescence analysis of U-2 OS cells using [KD Validated] Ku70 Rabbit polyclonal antibody (STJ11105550) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
