• Immunohistochemistry analysis of WSB2 in paraffin-embedded rat heart using WSB2 Rabbit polyclonal antibody (STJ114836) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using WSB2 Rabbit polyclonal antibody (STJ114836) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-WSB2 antibody (1-200) (STJ114836)

SKU:
STJ114836

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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WSB2 (1-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WSB2
Gene ID: 55884
Uniprot ID: WSB2_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human WSB2 (NP_061109.1).
Immunogen Sequence: MEAGEEPLLLAELKPGRPHQ FDWKSSCETWSVAFSPDGSW FAWSQGHCIVKLIPWPLEEQ FIPKGFEAKSRSSKNETKGR GSPKEKTLDCGQIVWGLAFS PWPSPPSRKLWARHHPQVPD VSCLVLATGLNDGQIKIWEV QTGLLLLNLSGHQDVVRDLS FTPSGSLILVSASRDKTLRI WDLNKHGKQIQVLSGHLQWV
Function May be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Protein Name Wd Repeat And Socs Box-Containing Protein 2
Wsb-2
Cs Box-Containing Wd Protein
Database Links Reactome: R-HSA-8951664
Alternative Antibody Names Anti-Wd Repeat And Socs Box-Containing Protein 2 antibody
Anti-Wsb-2 antibody
Anti-Cs Box-Containing Wd Protein antibody
Anti-WSB2 antibody

Information sourced from Uniprot.org

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