• Western blot analysis of extracts of mouse lung, using WNT9A antibody (STJ110248) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of mouse lung, using WNT9A antibody (STJ110248) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-WNT9A antibody (250-350) (STJ110248)

SKU:
STJ110248

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WNT9A (250-350) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WNT9A
Gene ID: 7483
Uniprot ID: WNT9A_HUMAN
Immunogen Region: 250-350
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 250-350 of human WNT9A (NP_003386.1).
Immunogen Sequence: ALKVGSTTNEAAGEAGAISP PRGRASGAGGSDPLPRTPEL VHLDDSPSFCLAGRFSPGTA GRRCHREKNCESICCGRGHN TQSRVVTRPCQCQVRWCCYV E
Post Translational Modifications Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.
Function Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal timing of IHH expression during embryonic bone development, normal chondrocyte maturation and for normal bone mineralization during embryonic bone development. Plays a redundant role in maintaining joint integrity.
Protein Name Protein Wnt-9a
Protein Wnt-14
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-3238698
Reactome: R-HSA-373080
Reactome: R-HSA-3772470
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Protein Wnt-9a antibody
Anti-Protein Wnt-14 antibody
Anti-WNT9A antibody
Anti-WNT14 antibody

Information sourced from Uniprot.org

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