Western blot analysis of extracts of various cell lines, using WNT2B Rabbit monoclonal antibody (STJ11103037) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-WNT2B antibody (200-300) [S7MR] (STJ11103037)

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STJ11103037
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:6000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WNT2B
Gene ID: 7482
Uniprot ID: WNT2B_HUMAN
Immunogen Region: 200-300
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human WNT2B (Q93097).
Immunogen Sequence: KAFVDAKEKRLKDARALMNL HNNRCGRTAVRRFLKLECKC HGVSGSCTLRTCWRALSDFR RTGDYLRRRYDGAVQVMATQ DGANFTAARQGYRRATRTDL V
Tissue Specificity Isoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidney. Isoform 2 is expressed in fetal brain, fetal lung, fetal kidney, caudate nucleus, testis and cancer cell lines.
Post Translational Modifications Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.
Function Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Plays a redundant role in embryonic lung development.
Protein Name Protein Wnt-2b
Protein Wnt-13
Database Links Reactome: R-HSA-3238698
Reactome: R-HSA-373080
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Protein Wnt-2b antibody
Anti-Protein Wnt-13 antibody
Anti-WNT2B antibody
Anti-WNT13 antibody

Information sourced from Uniprot.org

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