Immunofluorescence analysis of U-2 OS cells using WNT10B Rabbit polyclonal antibody (STJ119128) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-WNT10B antibody (260-340) (STJ119128)

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STJ119128
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WNT10B
Gene ID: 7480
Uniprot ID: WN10B_HUMAN
Immunogen Region: 260-340
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 260-340 of human WNT10B (NP_003385.2).
Immunogen Sequence: TCWRAAPEFRAVGAALRERL GRAIFIDTHNRNSGAFQPRL RPRRLSGELVYFEKSPDFCE RDPTMGSPGTRGRACNKTSR L
Tissue Specificity Detected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain.
Post Translational Modifications Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.
Function Member of the Wnt ligand gene family that encodes for secreted proteins, which activate the Wnt signaling cascade. Specifically activates canonical Wnt/beta-catenin signaling and thus triggers beta-catenin/LEF/TCF-mediated transcriptional programs. Involved in signaling networks controlling stemness, pluripotency and cell fate decisions. Acts in the immune system, mammary gland, adipose tissue, bone and skin.
Protein Name Protein Wnt-10b
Protein Wnt-12
Database Links Reactome: R-HSA-3238698
Reactome: R-HSA-373080
Reactome: R-HSA-381340
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Protein Wnt-10b antibody
Anti-Protein Wnt-12 antibody
Anti-WNT10B antibody
Anti-WNT12 antibody

Information sourced from Uniprot.org

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