Western blot analysis of extracts of various cell lines, using WISP1 antibody (STJ26112) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-WISP1 antibody (158-367) (STJ26112)

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STJ26112
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCN4
Gene ID: 8840
Uniprot ID: CCN4_HUMAN
Immunogen Region: 158-367
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 158-367 of human WISP1 (NP_003873.1).
Immunogen Sequence: LRVRPPRLWCPHPRRVSIPG HCCEQWVCEDDAKRPRKTAP RDTGAFDAVGEVEAWHRNCI AYTSPWSPCSTSCGLGVSTR ISNVNAQCWPEQESRLCNLR PCDVDIHTLIKAGKKCLAVY QPEASMNFTLAGCISTRSYQ PKYCGVCMDNRCCIPYKSKT IDVSFQCPDGLGFSRQVLWI NACFCNLSCRNPNDIFADLE SYPDFSEIAN
Tissue Specificity Expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Isoform 2 is expressed predominantly in scirrhous gastric carcinoma and, weakly in placenta. Overexpression is associated with several cancers including breast cancer and colon tumors. Isoform 2 is overexpressed in scirrhous gastric carcinoma.
Function Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan.
Protein Name Ccn Family Member 4
Wnt1-Inducible-Signaling Pathway Protein 1
Wisp-1
Wnt-1-Induced Secreted Protein
Cellular Localisation Secreted
Alternative Antibody Names Anti-Ccn Family Member 4 antibody
Anti-Wnt1-Inducible-Signaling Pathway Protein 1 antibody
Anti-Wisp-1 antibody
Anti-Wnt-1-Induced Secreted Protein antibody
Anti-CCN4 antibody
Anti-WISP1 antibody

Information sourced from Uniprot.org

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