• Western blot analysis of extracts of OVCAR3 cells, using HE4/WFDC2 antibody (STJ28871) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunofluorescence analysis of MCF7 cells using HE4/WFDC2 rabbit polyclonal antibody (STJ28871) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-WFDC2 antibody (31-124) (STJ28871)

SKU:
STJ28871

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WFDC2 (31-124) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WFDC2
Gene ID: 10406
Uniprot ID: WFDC2_HUMAN
Immunogen Region: 31-124
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 31-124 of human HE4/WFDC2 (NP_006094.3).
Immunogen Sequence: EKTGVCPELQADQNCTQECV SDSECADNLKCCSAGCATFC SLPNDKEGSCPQVNINFPQL GLCRDQCQVDSQCPGQMKCC RNGCGKVSCVTPNF
Tissue Specificity Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.
Function Broad range protease inhibitor.
Protein Name Wap Four-Disulfide Core Domain Protein 2
Epididymal Secretory Protein E4
Major Epididymis-Specific Protein E4
Putative Protease Inhibitor Wap5
Cellular Localisation Secreted
Alternative Antibody Names Anti-Wap Four-Disulfide Core Domain Protein 2 antibody
Anti-Epididymal Secretory Protein E4 antibody
Anti-Major Epididymis-Specific Protein E4 antibody
Anti-Putative Protease Inhibitor Wap5 antibody
Anti-WFDC2 antibody
Anti-HE4 antibody
Anti-WAP5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance