| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-WDR4 (20-250) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
WDR4 |
| Gene ID: |
10785 |
| Uniprot ID: |
WDR4_HUMAN |
| Immunogen Region: |
20-250 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 20-250 of human WDR4 (NP_061139.2). |
| Immunogen Sequence: |
SRFLATSIASSDDDSLFIYD CSAAEKKSQENKGEDAPLDQ GSGAILASTFSKSGSYFALT DDSKRLILFRTKPWQCLSVR TVARRCTALTFIASEEKVLV ADKSGDVYSFSVLEPHGCGR LELGHLSMLLDVAVSPDDRF ILTADRDEKIRVSWAAAPHS IESFCLGHTEFVSRISVVPT QPGLLLSSSGDGTLRLWEYR SGRQLHCCHLASLQELVDPQ APQKFAASRIA |
| Function | Non-catalytic component of the METTL1-WDR4 methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs). In the METTL1-WDR4 methyltransferase complex, WDR4 acts as a scaffold for tRNA-binding. Required for the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5'-RAGGU-3' motif within the variable loop. M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay. Also required for the formation of N(7)-methylguanine at internal sites in a subset of mRNAs. Also required for methylation of a specific subset of miRNAs, such as let-7. Independently of METTL1, also plays a role in genome stability: localizes at the DNA replication site and regulates endonucleolytic activities of FEN1. |
| Protein Name | TrnaGuanine-N(7--Methyltransferase Non-Catalytic Subunit Wdr4Protein Wuho HomologHwhWd Repeat-Containing Protein 4 |
| Database Links | Reactome: R-HSA-6782315 |
| Cellular Localisation | NucleusChromosomeLocalizes At The Site Of Nascent Dna Synthesis |
| Alternative Antibody Names | Anti-Trna antibodyAnti-Guanine-N(7--Methyltransferase Non-Catalytic Subunit Wdr4 antibodyAnti-Protein Wuho Homolog antibodyAnti-Hwh antibodyAnti-Wd Repeat-Containing Protein 4 antibodyAnti-WDR4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
