• Western blot analysis of lysates from LO2 cells, using WDR35 Rabbit polyclonal antibody (STJ114690) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-WDR35 antibody (871-1170) (STJ114690)

SKU:
STJ114690

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WDR35 (871-1170) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WDR35
Gene ID: 57539
Uniprot ID: WDR35_HUMAN
Immunogen Region: 871-1170
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 871-1170 of human WDR35 (NP_065830.2).
Immunogen Sequence: CSQPKAAVDTCVHLNQWNKA VELAKNHSMKEIGSLLARYA SHLLEKNKTLDAIELYRKAN YFFDAAKLMFKIADEEAKKG SKPLRVKKLYVLSALLIEQY HEQMKNAQRGKVKGKSSEAT SALAGLLEEEVLSTTDRFTD NAWRGAEAYHFFILAQRQLY EGCVDTALKTALHLKDYEDI IPPVEIYSLLALCACASRAF GTCSKAFIKLKSLETLSSEQ KQQYEDLALEIFTKHTSKD
Function As a component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs), it is involved in ciliogenesis and ciliary protein trafficking. May promote CASP3 activation and TNF-stimulated apoptosis.
Protein Name Wd Repeat-Containing Protein 35
Intraflagellar Transport Protein 121 Homolog
Database Links Reactome: R-HSA-5610787
Reactome: R-HSA-5620924
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Cilium Axoneme
Cilium Basal Body
Alternative Antibody Names Anti-Wd Repeat-Containing Protein 35 antibody
Anti-Intraflagellar Transport Protein 121 Homolog antibody
Anti-WDR35 antibody
Anti-IFT121 antibody
Anti-KIAA1336 antibody

Information sourced from Uniprot.org

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