Western blot analysis of extracts of various cell lines, using Tryptophanyl-tRNA synthetase 1 antibody (STJ28325) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-WARS antibody (1-270) (STJ28325)

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STJ28325
£112.50 - £524.50
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Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WARS1
Gene ID: 7453
Uniprot ID: SYWC_HUMAN
Immunogen Region: 1-270
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human Tryptophanyl-tRNA synthetase 1 (NP_776049.1).
Immunogen Sequence: MPNSEPASLLELFNSIATQG ELVRSLKAGNASKDEIDSAV KMLVSLKMSYKAAAGEDYKA DCPPGNPAPTSNHGPDATEA EEDFVDPWTVQTSSAKGIDY DKLIVRFGSSKIDKELINRI ERATGQRPHHFLRRGIFFSH RDMNQVLDAYENKKPFYLYT GRGPSSEAMHVGHLIPFIFT KWLQDVFNVPLVIQMTDDEK YLWKDLTLDQAYSYAVENAK DIIACGFDINKTFIFSDLD
Post Translational Modifications Proteolytic cleavage generates 2 forms.T1-TrpRS and T2-TrpRS.
Function Catalyzes the attachment of tryptophan to tRNA(Trp) in a two-step reaction: tryptophan is first activated by ATP to form Trp-AMP and then transferred to the acceptor end of the tRNA(Trp). Isoform 1: Has no angiostatic activity. T2-TrpRS: Possesses an angiostatic activity but has no aminoacylation activity. Inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. Isoform 2: Has an angiostatic activity.
Protein Name Tryptophan--Trna Ligase - Cytoplasmic
Interferon-Induced Protein 53
Ifp53
Tryptophanyl-Trna Synthetase
Trprs
Hwrs Cleaved Into - T1-Trprs - T2-Trprs
Database Links Reactome: R-HSA-379716
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Tryptophan--Trna Ligase - Cytoplasmic antibody
Anti-Interferon-Induced Protein 53 antibody
Anti-Ifp53 antibody
Anti-Tryptophanyl-Trna Synthetase antibody
Anti-Trprs antibody
Anti-Hwrs Cleaved Into - T1-Trprs - T2-Trprs antibody
Anti-WARS1 antibody
Anti-IFI53 antibody
Anti-WARS antibody
Anti-WRS antibody

Information sourced from Uniprot.org

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