• Western blot analysis of lysates from HepG2 cells, using VRK3 Rabbit polyclonal antibody (STJ11101528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-VRK3 antibody (266-412) (STJ11101528)

SKU:
STJ11101528

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-VRK3 (266-412) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VRK3
Gene ID: 51231
Uniprot ID: VRK3_HUMAN
Immunogen Region: 266-412
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 266-412 of human VRK3 (NP_001295349).
Immunogen Sequence: LQSALDVSPKHVLSERSVLQ VACRLLDALEFLHENEYVHG NVTAENIFVDPEDQSQVTLA GYGFAFRYCPSGKHVAYVEG SRSPHEGDLEFISMDLHKGC GPSRRSDLQSLGYCMLKWLY GFLPWTNCLPNTEDIMKQKQ KLPWDSF
Function Inactive kinase that suppresses ERK activity by promoting phosphatase activity of DUSP3 which specifically dephosphorylates and inactivates ERK in the nucleus.
Protein Name Inactive Serine/Threonine-Protein Kinase Vrk3
Serine/Threonine-Protein Pseudokinase Vrk3
Vaccinia-Related Kinase 3
Database Links Reactome: R-HSA-202670
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Inactive Serine/Threonine-Protein Kinase Vrk3 antibody
Anti-Serine/Threonine-Protein Pseudokinase Vrk3 antibody
Anti-Vaccinia-Related Kinase 3 antibody
Anti-VRK3 antibody

Information sourced from Uniprot.org

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