| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | VAPB |
| Gene ID: | 9217 |
| Uniprot ID: | VAPB_HUMAN |
| Immunogen Region: | 10-243 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 10-222 of human VAPB (NP_004729.1). |
| Immunogen Sequence: | LEPQHELKFRGPFTDVVTTN LKLGNPTDRNVCFKVKTTAP RRYCVRPNSGIIDAGASINV SVMLQPFDYDPNEKSKHKFM VQSMFAPTDTSDMEAVWKEA KPEDLMDSKLRCVFELPAEN DKPHDVEINKIISTTASKTE TPIVSKSLSSSLDDTEVKKV MEECKRLQGEVQRLREENKQ FKEEDGLRMRKTVQSNSPIS ALAPTGKEEGLST |
| Tissue Specificity | Ubiquitous. Isoform 1 predominates. |
| Function | Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44. Interacts with STARD3 in a FFAT motif phosphorylation dependent manner. Via interaction with WDR44 participates in neosynthesized protein export. Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. Involved in cellular calcium homeostasis regulation. |
| Protein Name | Vesicle-Associated Membrane Protein-Associated Protein B/CVamp-B/Vamp-CVamp-Associated Protein B/CVap-B/Vap-C |
| Database Links | Reactome: R-HSA-1660661Reactome: R-HSA-8980692Reactome: R-HSA-9013106Reactome: R-HSA-9013404Reactome: R-HSA-9013405Reactome: R-HSA-9013408 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type Iv Membrane ProteinPresent In Mitochondria-Associated Membranes That Are Endoplasmic Reticulum Membrane Regions Closely Apposed To The Outer Mitochondrial Membrane |
| Alternative Antibody Names | Anti-Vesicle-Associated Membrane Protein-Associated Protein B/C antibodyAnti-Vamp-B/Vamp-C antibodyAnti-Vamp-Associated Protein B/C antibodyAnti-Vap-B/Vap-C antibodyAnti-VAPB antibodyAnti-UNQ484 antibodyAnti-PRO983 antibody |
Information sourced from Uniprot.org