• Western blot analysis of various lysates, using VAMP7 antibody (STJ11101489) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
  • Western blot analysis of mouse brain, using VAMP7 antibody (STJ11101489) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunofluorescence analysis of U2OS cells using VAMP7 antibody (STJ11101489) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using VAMP7 antibody (STJ11101489) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using VAMP7 antibody (STJ11101489) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-VAMP7 antibody (11-70) (STJ11101489)

SKU:
STJ11101489

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-VAMP7 (11-70) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VAMP7
Gene ID: 6845
Uniprot ID: VAMP7_HUMAN
Immunogen Region: 11-70
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 11-70 of human VAMP7 (P51809).
Immunogen Sequence: GTTILAKHAWCGGNFLEVTE QILAKIPSENNKLTYSHGNY LFHYICQDRIVYLCITDDDF
Tissue Specificity Detected in all tissues tested.
Function Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.
Protein Name Vesicle-Associated Membrane Protein 7
Vamp-7
Synaptobrevin-Like Protein 1
Tetanus-Insensitive Vamp
Ti-Vamp
Database Links Reactome: R-HSA-199992
Reactome: R-HSA-432720
Reactome: R-HSA-432722
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-9020591
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle Membrane
Single-Pass Type Iv Membrane Protein
Golgi Apparatus
Trans-Golgi Network Membrane
Late Endosome Membrane
Lysosome Membrane
Endoplasmic Reticulum Membrane
Phagosome Membrane
Synapse
Synaptosome
In Immature Neurons Expression Is Localized In Vesicular Structures In Axons And Dendrites While In Mature Neurons It Is Localized To The Somatodendritic Region
Colocalizes With Lamp1 In Kidney Cells
Localization To The Endoplasmic Reticulum Membrane Was Observed In The Intestine But Not In Liver Or Kidney
Alternative Antibody Names Anti-Vesicle-Associated Membrane Protein 7 antibody
Anti-Vamp-7 antibody
Anti-Synaptobrevin-Like Protein 1 antibody
Anti-Tetanus-Insensitive Vamp antibody
Anti-Ti-Vamp antibody
Anti-VAMP7 antibody
Anti-SYBL1 antibody

Information sourced from Uniprot.org

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