| Host: |
NZ White Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Nz White Rabbit polyclonal antibody anti-VAMP A/B (50-100) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Shipped as lyophilised. Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material. |
| Purification: |
Ammonium sulphate precipitation |
| Dilution Range: |
A concentration of 10-50 µg/ml of is recommended. The optimal concentration should be determined by the end user. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Immunogen Region: |
50-100 |
| Specificity: |
Specific for VAMP A and B. |
| Immunogen: |
A synthetic peptide from aa region 50-100 of human VAMPB conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in many species including human, rat, mouse, zebra fish, bovine, xenopus and chicken. |
| Post Translational Modifications | Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamate increases conductance by 40%.this increase is not due to increased presence at the cell membrane. Isoform 2: Palmitoylated on its N-terminal region. Isoform 1: Not palmitoylated. |
| Function | Forms a water-specific channel. Plays an important role in brain water homeostasis and in glymphatic solute transport. Required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability. |
| Protein Name | Aquaporin-4Aqp-4Mercurial-Insensitive Water ChannelMiwcWch4 |
| Database Links | Reactome: R-MMU-432040Reactome: -MMU-432047 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneEndosome MembraneSarcolemmaCell ProjectionActivation Of The Vasopressin Receptor Avpr1a Triggers Aqp4 Phosphorylation At Ser-180 And Promotes Its Internalization From The Cell MembraneDetected On Brain Astrocyte Processes And Astrocyte Endfeet Close To Capillaries |
| Alternative Antibody Names | Anti-Aquaporin-4 antibodyAnti-Aqp-4 antibodyAnti-Mercurial-Insensitive Water Channel antibodyAnti-Miwc antibodyAnti-Wch4 antibodyAnti-Aqp4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
