• Western blot analysis of lysates from K-562 cells, using USP3 Rabbit polyclonal antibody (STJ118214) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-USP3 antibody (321-520) (STJ118214)

SKU:
STJ118214

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USP3
Gene ID: 9960
Uniprot ID: UBP3_HUMAN
Immunogen Region: 321-520
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 321-520 of human USP3 (NP_006528.2).
Immunogen Sequence: EVNCLICGTESRKFDPFLDL SLDIPSQFRSKRSKNQENGP VCSLRDCLRSFTDLEELDET ELYMCHKCKKKQKSTKKFWI QKLPKVLCLHLKRFHWTAYL RNKVDTYVEFPLRGLDMKCY LLEPENSGPESCLYDLAAVV VHHGSGVGSGHYTAYATHEG RWFHFNDSTVTLTDEETVVK AKAYILFYVEHQAKAGSDKL
Tissue Specificity Expressed in all tissues examined, with strongest expression in pancreas.
Function Deubiquitinase that plays a role in several cellular processes including transcriptional regulation, cell cycle progression or innate immunity. In response to DNA damage, deubiquitinates monoubiquitinated target proteins such as histone H2A and H2AX and thereby counteracts RNF168- and RNF8-mediated ubiquitination. In turn, participates in the recruitment of DNA damage repair factors to DNA break sites. Required for proper progression through S phase and subsequent mitotic entry. Acts as a positive regulator of TP53 by deubiquitinating and stabilizing it to promote normal cell proliferation and transformation. Participates in establishing tolerance innate immune memory through non-transcriptional feedback. Mechanistically, negatively regulates TLR-induced NF-kappa-B signaling by targeting and removing the 'Lys-63'-linked polyubiquitin chains on MYD88. Negatively regulates the activation of type I interferon signaling by mediating 'Lys-63'-linked polyubiquitin chains on RIGI and IFIH1. Deubiquitinates also ASC/PYCARD, the central adapter mediating the assembly and activation of most inflammasomes, and thereby promotes inflammasome activation.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase 3
Deubiquitinating Enzyme 3
Ubiquitin Thioesterase 3
Ubiquitin-Specific-Processing Protease 3
Database Links Reactome: R-HSA-5689880
Cellular Localisation Nucleus
Cytoplasm
Localizes Preferentially With Monoubiquitinated H2a To Chromatin
Upon Nf-Kappa-B Signaling Activation
Exits The Nucleus
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase 3 antibody
Anti-Deubiquitinating Enzyme 3 antibody
Anti-Ubiquitin Thioesterase 3 antibody
Anti-Ubiquitin-Specific-Processing Protease 3 antibody
Anti-USP3 antibody

Information sourced from Uniprot.org