• Western blot analysis of lysates from K-562 cells, using USP3 Rabbit polyclonal antibody (STJ118214) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-USP3 antibody (321-520) (STJ118214)

SKU:
STJ118214

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-USP3 (321-520) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USP3
Gene ID: 9960
Uniprot ID: UBP3_HUMAN
Immunogen Region: 321-520
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 321-520 of human USP3 (NP_006528.2).
Immunogen Sequence: EVNCLICGTESRKFDPFLDL SLDIPSQFRSKRSKNQENGP VCSLRDCLRSFTDLEELDET ELYMCHKCKKKQKSTKKFWI QKLPKVLCLHLKRFHWTAYL RNKVDTYVEFPLRGLDMKCY LLEPENSGPESCLYDLAAVV VHHGSGVGSGHYTAYATHEG RWFHFNDSTVTLTDEETVVK AKAYILFYVEHQAKAGSDKL
Tissue Specificity Expressed in all tissues examined, with strongest expression in pancreas.
Function Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase 3
Deubiquitinating Enzyme 3
Ubiquitin Thioesterase 3
Ubiquitin-Specific-Processing Protease 3
Database Links Reactome: R-HSA-5689880
Cellular Localisation Nucleus
Localizes Preferentially With Monoubiquitinated H2a To Chromatin
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase 3 antibody
Anti-Deubiquitinating Enzyme 3 antibody
Anti-Ubiquitin Thioesterase 3 antibody
Anti-Ubiquitin-Specific-Processing Protease 3 antibody
Anti-USP3 antibody

Information sourced from Uniprot.org

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