| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | USP3 |
| Gene ID: | 9960 |
| Uniprot ID: | UBP3_HUMAN |
| Immunogen Region: | 321-520 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 321-520 of human USP3 (NP_006528.2). |
| Immunogen Sequence: | EVNCLICGTESRKFDPFLDL SLDIPSQFRSKRSKNQENGP VCSLRDCLRSFTDLEELDET ELYMCHKCKKKQKSTKKFWI QKLPKVLCLHLKRFHWTAYL RNKVDTYVEFPLRGLDMKCY LLEPENSGPESCLYDLAAVV VHHGSGVGSGHYTAYATHEG RWFHFNDSTVTLTDEETVVK AKAYILFYVEHQAKAGSDKL |
| Tissue Specificity | Expressed in all tissues examined, with strongest expression in pancreas. |
| Function | Deubiquitinase that plays a role in several cellular processes including transcriptional regulation, cell cycle progression or innate immunity. In response to DNA damage, deubiquitinates monoubiquitinated target proteins such as histone H2A and H2AX and thereby counteracts RNF168- and RNF8-mediated ubiquitination. In turn, participates in the recruitment of DNA damage repair factors to DNA break sites. Required for proper progression through S phase and subsequent mitotic entry. Acts as a positive regulator of TP53 by deubiquitinating and stabilizing it to promote normal cell proliferation and transformation. Participates in establishing tolerance innate immune memory through non-transcriptional feedback. Mechanistically, negatively regulates TLR-induced NF-kappa-B signaling by targeting and removing the 'Lys-63'-linked polyubiquitin chains on MYD88. Negatively regulates the activation of type I interferon signaling by mediating 'Lys-63'-linked polyubiquitin chains on RIGI and IFIH1. Deubiquitinates also ASC/PYCARD, the central adapter mediating the assembly and activation of most inflammasomes, and thereby promotes inflammasome activation. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 3 Deubiquitinating Enzyme 3 Ubiquitin Thioesterase 3 Ubiquitin-Specific-Processing Protease 3 |
| Database Links | Reactome: R-HSA-5689880 |
| Cellular Localisation | Nucleus Cytoplasm Localizes Preferentially With Monoubiquitinated H2a To Chromatin Upon Nf-Kappa-B Signaling Activation Exits The Nucleus |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 3 antibody Anti-Deubiquitinating Enzyme 3 antibody Anti-Ubiquitin Thioesterase 3 antibody Anti-Ubiquitin-Specific-Processing Protease 3 antibody Anti-USP3 antibody |
Information sourced from Uniprot.org
