• Immunofluorescence analysis of HeLa cells using UNG Rabbit polyclonal antibody (STJ115121) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using UNG Rabbit polyclonal antibody (STJ115121) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-UNG antibody (1-100) (STJ115121)

SKU:
STJ115121

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UNG (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UNG
Gene ID: 7374
Uniprot ID: UNG_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UNG (NP_550433.1).
Immunogen Sequence: MIGQKTLYSFFSPSPARKRH APSPEPAVQGTGVAGVPEES GDAAAIPAKKAPAGQEEPGT PPSSPLSAEQLDRIQRNKAA ALLRLAARNVPVGFGESWKK
Tissue Specificity Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.
Post Translational Modifications Isoform 1 is processed by cleavage of a transit peptide.
Function Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
Protein Name Uracil-Dna Glycosylase
Udg
Database Links Reactome: R-HSA-110328 P13051-1
Reactome: R-HSA-110329 P13051-1
Reactome: R-HSA-110357 P13051-1
Cellular Localisation Isoform 1: Mitochondrion
Isoform 2: Nucleus
Alternative Antibody Names Anti-Uracil-Dna Glycosylase antibody
Anti-Udg antibody
Anti-UNG antibody
Anti-DGU antibody
Anti-UNG1 antibody
Anti-UNG15 antibody

Information sourced from Uniprot.org

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