• Western blot analysis of extracts of various cell lines, using UBA3 antibody (STJ29640) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-UBA3 antibody (234-463) (STJ29640)

SKU:
STJ29640

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UBA3 (234-463) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBA3
Gene ID: 9039
Uniprot ID: UBA3_HUMAN
Immunogen Region: 234-463
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 234-463 of human UBA3 (NP_003959.3).
Immunogen Sequence: FPMCTIASMPRLPEHCIEYV RMLQWPKEQPFGEGVPLDGD DPEHIQWIFQKSLERASQYN IRGVTYRLTQGVVKRIIPAV ASTNAVIAAVCATEVFKIAT SAYIPLNNYLVFNDVDGLYT YTFEAERKENCPACSQLPQN IQFSPSAKLQEVLDYLTNSA SLQMKSPAITATLEGKNRTL YLQSVTSIEERTRPNLSKTL KELGLVDGQELAVADVTTPQ TVLFKLHFTS
Tissue Specificity Ubiquitously expressed.
Function Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.
Protein Name Nedd8-Activating Enzyme E1 Catalytic Subunit
Nedd8-Activating Enzyme E1c
Ubiquitin-Activating Enzyme E1c
Ubiquitin-Like Modifier-Activating Enzyme 3
Ubiquitin-Activating Enzyme 3
Database Links Reactome: R-HSA-5607761
Reactome: R-HSA-5676590
Reactome: R-HSA-8951664
Reactome: R-HSA-983168
Alternative Antibody Names Anti-Nedd8-Activating Enzyme E1 Catalytic Subunit antibody
Anti-Nedd8-Activating Enzyme E1c antibody
Anti-Ubiquitin-Activating Enzyme E1c antibody
Anti-Ubiquitin-Like Modifier-Activating Enzyme 3 antibody
Anti-Ubiquitin-Activating Enzyme 3 antibody
Anti-UBA3 antibody
Anti-UBE1C antibody

Information sourced from Uniprot.org

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