• Immunofluorescence analysis of HeLa cells using Thioredoxin 1 (Trx1/Thioredoxin 1 (Trx1/TXN) ) Rabbit polyclonal antibody (STJ116993) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded Mouse testis using Thioredoxin 1 (Trx1/Thioredoxin 1 (Trx1/TXN) ) antibody (STJ116993) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Mouse lung using Thioredoxin 1 (Trx1/Thioredoxin 1 (Trx1/TXN) ) antibody (STJ116993) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human lung using Thioredoxin 1 (Trx1/Thioredoxin 1 (Trx1/TXN) ) antibody (STJ116993) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Rat heart using Thioredoxin 1 (Trx1/Thioredoxin 1 (Trx1/TXN) ) antibody (STJ116993) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Rat brain using Thioredoxin 1 (Trx1/Thioredoxin 1 (Trx1/TXN) ) antibody (STJ116993) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Rat lung using Thioredoxin 1 (Trx1/Thioredoxin 1 (Trx1/TXN) ) antibody (STJ116993) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Thioredoxin 1 (Trx1/Thioredoxin 1 (Trx1/TXN) ) antibody (STJ116993) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TXN antibody (2-105) (STJ116993)

SKU:
STJ116993

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TXN (2-105) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TXN
Gene ID: 7295
Uniprot ID: THIO_HUMAN
Immunogen Region: 2-105
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 2-105 of human Trx1/Thioredoxin 1 (Trx1/TXN) (NP_003320.2).
Immunogen Sequence: VKQIESKTAFQEALDAAGDK LVVVDFSATWCGPCKMIKPF FHSLSEKYSNVIFLEVDVDD CQDVASECEVKCMPTFQFFK KGQKVGEFSGANKEKLEATI NELV
Post Translational Modifications In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins. In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
Function Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
Protein Name Thioredoxin
Trx
Atl-Derived Factor
Adf
Surface-Associated Sulphydryl Protein
Sasp
Allergen Hom S Trx
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-3299685
Reactome: R-HSA-499943
Reactome: R-HSA-5628897
Reactome: R-HSA-5676934
Reactome: R-HSA-844456
Reactome: R-HSA-9617629
Reactome: R-HSA-9660826
Cellular Localisation Nucleus
Cytoplasm
Secreted
Translocates From The Cytoplasm Into The Nucleus After Phorbol 12-Myristate 13-Acetate Induction (Pma)
Predominantly In The Cytoplasm In Non Irradiated Cells
Radiation Induces Translocation Of Trx From The Cytoplasm To The Nucleus
Secreted By A Leaderless Secretory Pathway
Alternative Antibody Names Anti-Thioredoxin antibody
Anti-Trx antibody
Anti-Atl-Derived Factor antibody
Anti-Adf antibody
Anti-Surface-Associated Sulphydryl Protein antibody
Anti-Sasp antibody
Anti-Allergen Hom S Trx antibody
Anti-TXN antibody
Anti-TRDX antibody
Anti-TRX antibody
Anti-TRX1 antibody

Information sourced from Uniprot.org

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