| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TXK (1-180) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TXK |
| Gene ID: |
7294 |
| Uniprot ID: |
TXK_HUMAN |
| Immunogen Region: |
1-180 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human TXK (NP_003319.2). |
| Immunogen Sequence: |
MILSSYNTIQSVFCCCCCCS VQKRQMRTQISLSTDEELPE KYTQRRRPWLSQLSNKKQSN TGRVQPSKRKPLPPLPPSEV AEEKIQVKALYDFLPREPCN LALRRAEEYLILEKYNPHWW KARDRLGNEGLIPSNYVTEN KITNLEIYEWYHRNITRNQA EHLLRQESKEGAFIVRDSRH |
| Tissue Specificity | Expressed in T-cells and some myeloid cell lines. Expressed in Th1/Th0 cells with IFN-gamma-producing potential. |
| Post Translational Modifications | Phosphorylated at Tyr-420 by FYN. Autophosphorylation at Tyr-91 is critical for the activation of TXK, leading to the up-regulation of IFN-gamma gene transcription. The cysteine string at the N-terminus is palmitoylated and required for the proper subcellular location. |
| Function | Non-receptor tyrosine kinase that plays a redundant role with ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation leads to the recruitment of TXK to the cell membrane, where it is phosphorylated at Tyr-420. Phosphorylation leads to TXK full activation. Contributes also to signaling from many receptors and participates in multiple downstream pathways, including regulation of the actin cytoskeleton. Like ITK, can phosphorylate PLCG1, leading to its localization in lipid rafts and activation, followed by subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with PARP1 and EEF1A1. Within the complex, phosphorylates both PARP1 and EEF1A1. Phosphorylates also key sites in LCP2 leading to the up-regulation of Th1 preferred cytokine IL-2. Phosphorylates 'Tyr-201' of CTLA4 which leads to the association of PI-3 kinase with the CTLA4 receptor. |
| Protein Name | Tyrosine-Protein Kinase TxkProtein-Tyrosine Kinase 4Resting Lymphocyte Kinase |
| Database Links | Reactome: R-HSA-2871809 |
| Cellular Localisation | CytoplasmNucleusCell MembranePeripheral Membrane ProteinLocalizes In The Vicinity Of Cell Surface Receptors In The Plasma Membrane After Receptor StimulationTranslocates Into The Nucleus And Enhances Ifn-Gamma Gene Transcription In T-Cells |
| Alternative Antibody Names | Anti-Tyrosine-Protein Kinase Txk antibodyAnti-Protein-Tyrosine Kinase 4 antibodyAnti-Resting Lymphocyte Kinase antibodyAnti-TXK antibodyAnti-PTK4 antibodyAnti-RLK antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
