| Host: |
Mouse |
| Applications: |
IHC-F/FC/ELISA/IP/WB |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Tumor Necrosis Factor Receptor is suitable for use in Immunohistochemistry, Flow Cytometry, ELISA, Immunoprecipitation and Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
H398 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG2a |
| Formulation: |
PBS with 0.1% BSA and 0.02% sodium azide |
| Concentration: |
100 ug/mL |
| Storage Instruction: |
Store at 2-8°C upon receipt. |
| Background | The monoclonal antibody H398 recognizes the extracellular part of the Tumor Necrosis Factor Receptor type I (TNF-RI) of the membrane-bound as well as the soluble receptor. TNF-RI (~55-60 kDa) is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNF-RI and TNF-RII, which have both distinct and overlapping downstream signaling cascades. Induction of cytotoxicity and other functions are mediated largely via TNF-RI. TNF-RI is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-RII is efficiently activated only by the membrane bound form of TNF-alpha. TNF-RI signaling is initiated when trimeric TNF-alpha binds TNF-RI receptors. Subsequent TNF-RI trimerization promotes the recruitment of a proximal signaling complex composed of TNF Receptor Associated protein with a Death Domain (TRADD) , Receptor Interacting Protein (RIP) , cellular Inhibitor of Apoptosis Protein 1 (cIAP1) , TNF Receptor Associated Factor 2 (TRAF2) , and likely TRAF5. Studies with TNF-RI-deficient mice indicate that TNF-RI mediates most of the proliferation, pro-inflammatory, and apoptosis-activating pathways. |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
