• Western blot analysis of extracts of various cell lines, using Gamma-Tubulin antibody (STJ116765) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TUBG1 antibody (350-450) (STJ116765)

SKU:
STJ116765

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Gamma-Tubulin (350-450) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TUBG1
Gene ID: 7283
Uniprot ID: TBG1_HUMAN
Immunogen Region: 350-450
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-450 of human Gamma-Tubulin (NP_001061.2).
Immunogen Sequence: PWGPASIQVALSRKSPYLPS AHRVSGLMMANHTSISSLFE RTCRQYDKLRKREAFLEQFR KEDMFKDNFDEMDTSREIVQ QLIDEYHAATRPDYISWGTQ E
Post Translational Modifications Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
Function Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.
Protein Name Tubulin Gamma-1 Chain
Gamma-1-Tubulin
Gamma-Tubulin Complex Component 1
Gcp-1
Database Links Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-8854518
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle
Localizes To Mitotic Spindle Microtubules
Alternative Antibody Names Anti-Tubulin Gamma-1 Chain antibody
Anti-Gamma-1-Tubulin antibody
Anti-Gamma-Tubulin Complex Component 1 antibody
Anti-Gcp-1 antibody
Anti-TUBG1 antibody
Anti-TUBG antibody

Information sourced from Uniprot.org

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