• Western blot analysis of extracts of various cell lines, using TSPYL1 antibody (STJ114067) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunohistochemistry of paraffin-embedded human liver cancer using TSPYL1 rabbit polyclonal antibody (STJ114067) at dilution of 1:400 (40x lens).
  • Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug TSPYL1 antibody (STJ114067). Western blot was performed from the immunoprecipitate using TSPYL1 antibody (STJ114067) at a dilition of 1:1000.

Anti-TSPYL1 antibody (1-230) (STJ114067)

SKU:
STJ114067

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TSPYL1 (1-230) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:500
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TSPYL1
Gene ID: 7259
Uniprot ID: TSYL1_HUMAN
Immunogen Region: 1-230
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human TSPYL1 (NP_003300.1).
Immunogen Sequence: MSGLDGVKRTTPLQTHSIII SDQVPSDQDAHQYLRLRDQS EATQVMAEPGEGGSETVALP PPPPSEEGGVPQDAAGRGGT PQIRVVGGRGHVAIKAGQEE GQPPAEGLAAASVVMAADRS LKKGVQGGEKALEICGAQRS ASELTAGAEAEAEEVKTGKC ATVSAAVAERESAEVVKEGL AEKEVMEEQMEVEEQPPEGE EIEVAEEDRLEEEAREEEGP WPLHEALRMD
Tissue Specificity Expressed in testis, ovary, liver, spleen, brain, kidney, prostate, lung, liver, and heart.
Post Translational Modifications Ubiquitinated by the CRL2(APPBP2) complex, which recognizes the Arg-Xaa-Xaa-Gly sequence at the C-terminus, leading to its degradation.
Protein Name Testis-Specific Y-Encoded-Like Protein 1
Tspy-Like Protein 1
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Testis-Specific Y-Encoded-Like Protein 1 antibody
Anti-Tspy-Like Protein 1 antibody
Anti-TSPYL1 antibody
Anti-TSPYL antibody

Information sourced from Uniprot.org

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