![Western blot analysis of lysates from wild type (WT) and TRMT2A/HTF9C knockout (KO) HeLa cells, using [KO Validated] TRMT2A/HTF9C Rabbit polyclonal antibody (STJ11100863) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89797/358266/STJ11100863_1__40910.1713122607.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TRMT2A/HTF9C knockout (KO) HeLa cells, using [KO Validated] TRMT2A/HTF9C Rabbit polyclonal antibody (STJ11100863) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western blot analysis of lysates from wild type (WT) and TRMT2A/HTF9C knockout (KO) HeLa cells, using [KO Validated] TRMT2A/HTF9C Rabbit polyclonal antibody (STJ11100863) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89797/358266/STJ11100863_1__40910.1713122607.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TRMT2A/HTF9C knockout (KO) HeLa cells, using [KO Validated] TRMT2A/HTF9C Rabbit polyclonal antibody (STJ11100863) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TRMT2A |
| Gene ID: | 27037 |
| Uniprot ID: | TRM2A_HUMAN |
| Immunogen Region: | 500 aa |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human TRMT2A/HTF9C (NP_073564.3). |
| Immunogen Sequence: | LASQHLVAILDPPRAGLHSK VILAIRRAKNLRRLLYVSCN PRAAMGNFVDLCRAPSNRVK GIPFRPVKAVAVDLFPQTPH CEMLILFERVEHPNGTGVLG PHSPPAQPTPGPPDNTLQET GTFPSS |
| Function | S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine in tRNAs and some mRNAs. Mainly catalyzes the methylation of uridine at position 54 (m5U54) in cytosolic tRNAs. Also able to mediate the formation of 5-methyl-uridine in some mRNAs. |
| Protein Name | TrnaUracil-5--Methyltransferase Homolog AMrnaUracil-5--Methyltransferase Trmt2a |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Trna antibodyAnti-Uracil-5--Methyltransferase Homolog A antibodyAnti-Mrna antibodyAnti-Uracil-5--Methyltransferase Trmt2a antibodyAnti-TRMT2A antibody |
Information sourced from Uniprot.org