| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-E3 ubiquitin/ISG15 ligase TRIM25 (201-250 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:10000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TRIM25 |
| Gene ID: | 7706 |
| Uniprot ID: | TRI25_HUMAN |
| Immunogen Region: | 201-250 aa |
| Specificity: | EFP Polyclonal Antibody detects endogenous levels of EFP protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human ZNF147 at the amino acid range 201-250 |
| Post Translational Modifications | Auto-ISGylated. (Microbial infection) Autoubiquitinated.promoted by Epstein-Barr virus protein BPLF1. |
| Function | Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of RIGI and IFIH1. Mediates 'Lys-63'-linked polyubiquitination of the RIGI N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection. Mediates 'Lys-63'-linked polyubiquitination of IFIH1. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3. Plays a role in promoting the restart of stalled replication forks via interaction with the KHDC3L-OOEP scaffold and subsequent ubiquitination of BLM, resulting in the recruitment and retainment of BLM at DNA replication forks. Plays an essential role in the antiviral activity of ZAP/ZC3HAV1.an antiviral protein which inhibits the replication of certain viruses. Mechanistically, mediates 'Lys-63'-linked polyubiquitination of ZAP/ZC3HAV1 that is required for its optimal binding to target mRNA. Mediates also the ubiquitination of various substrates implicated in stress granule formation, nonsense-mediated mRNA decay, nucleoside synthesis and mRNA translation and stability. |
| Protein Name | E3 Ubiquitin/Isg15 Ligase Trim25 Estrogen-Responsive Finger Protein Ring Finger Protein 147 Ring-Type E3 Ubiquitin Transferase Ring-Type E3 Ubiquitin Transferase Trim25 Tripartite Motif-Containing Protein 25 Ubiquitin/Isg15-Conjugating Enzyme Trim25 Zinc Finger Protein 147 |
| Database Links | Reactome: R-HSA-1169408 Reactome: R-HSA-168928 Reactome: R-HSA-5656169 Reactome: R-HSA-5689896 Reactome: R-HSA-877300 Reactome: R-HSA-918233 Reactome: R-HSA-933541 Reactome: R-HSA-933542 Reactome: R-HSA-933543 Reactome: R-HSA-936440 Reactome: R-HSA-9692916 Reactome: R-HSA-9705671 Reactome: R-HSA-9833109 Reactome: R-HSA-9833110 Reactome: R-HSA-9833482 |
| Cellular Localisation | Cytoplasm Stress Granule Nucleus |
| Alternative Antibody Names | Anti-E3 Ubiquitin/Isg15 Ligase Trim25 antibody Anti-Estrogen-Responsive Finger Protein antibody Anti-Ring Finger Protein 147 antibody Anti-Ring-Type E3 Ubiquitin Transferase antibody Anti-Ring-Type E3 Ubiquitin Transferase Trim25 antibody Anti-Tripartite Motif-Containing Protein 25 antibody Anti-Ubiquitin/Isg15-Conjugating Enzyme Trim25 antibody Anti-Zinc Finger Protein 147 antibody Anti-TRIM25 antibody Anti-EFP antibody Anti-RNF147 antibody Anti-ZNF147 antibody |
Information sourced from Uniprot.org