||WB, IHC, IF, IP
||Human, Mouse, Rat
||FOR RESEARCH USE ONLY (RUO).
||Rabbit polyclonal antibody anti-TRIM21 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation.
||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
||WB 1:500-1:2000IHC 1:50-1:200IF 1:50-1:200IP 1:20-1:50
||Store at-20°C. Avoid freeze/thaw cycles.
||Recombinant fusion protein containing a sequence corresponding to amino acids 80-330 of human TRIM21 (NP_003132.2).
| Tissue Specificity || Isoform 1 and isoform 2 are expressed in fetal and adult heart and fetal lung. |
| Post Translational Modifications || Autoubiquitinated.does not lead to its proteasomal degradation. Deubiquitinated by USP4.leading to its stabilization. |
| Function || E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1 and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of IRF3, hence attenuating type I interferon (IFN)-dependent immune responses. Represses the innate antiviral response by facilitating the formation of the NMI-IFI35 complex through 'Lys-63'-linked ubiquitination of NMI. |
| Protein Name || E3 Ubiquitin-Protein Ligase Trim2152 Kda Ro Protein52 Kda Ribonucleoprotein Autoantigen Ro/Ss-ARing Finger Protein 81Ring-Type E3 Ubiquitin Transferase Trim21Ro(Ss-ASjoegren Syndrome Type A AntigenSs-ATripartite Motif-Containing Protein 21 |
| Database Links || Reactome: R-HSA-1834941Reactome: R-HSA-3134975Reactome: R-HSA-877300Reactome: R-HSA-983168 |
| Cellular Localisation || CytoplasmCytoplasmic VesicleAutophagosomeNucleusP-BodyEnters The Nucleus Upon Exposure To Nitric OxideLocalizes To Small Dot- Or Rod-Like Structures In The CytoplasmCalled Processing Bodies (P-Bodies) That Are Located Underneath The Plasma Membrane And Also Diffusely In The CytoplasmThey Are Located Along The Microtubules And Are Highly Motile In CellsColocalizes With Dcp2 In P-Bodies |
| Alternative Antibody Names || Anti-E3 Ubiquitin-Protein Ligase Trim21 antibodyAnti-52 Kda Ro Protein antibodyAnti-52 Kda Ribonucleoprotein Autoantigen Ro/Ss-A antibodyAnti-Ring Finger Protein 81 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Trim21 antibodyAnti-Ro(Ss-A antibodyAnti-Sjoegren Syndrome Type A Antigen antibodyAnti-Ss-A antibodyAnti-Tripartite Motif-Containing Protein 21 antibodyAnti-TRIM21 antibodyAnti-RNF81 antibodyAnti-RO52 antibodyAnti-SSA1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance