• Western blot analysis of extracts of various cell lines, using TRIM21 antibody (STJ115508) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunohistochemistry of paraffin-embedded mouse spinal cord using TRIM21 rabbit polyclonal antibody (STJ115508) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat kidney using TRIM21 rabbit polyclonal antibody (STJ115508) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using TRIM21 rabbit polyclonal antibody (STJ115508) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

Anti-TRIM21 antibody (80-330) (STJ115508)


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Host: Rabbit
Applications: WB, IHC, IF, IP
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal antibody anti-TRIM21 (80-330) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
IF 1:50-1:200
IP 1:20-1:50
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: TRIM21
Gene ID: 6737
Uniprot ID: RO52_HUMAN
Immunogen Region: 80-330
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-330 of human TRIM21 (NP_003132.2).
Tissue Specificity Isoform 1 and isoform 2 are expressed in fetal and adult heart and fetal lung.
Post Translational Modifications Autoubiquitinated.does not lead to its proteasomal degradation. Deubiquitinated by USP4.leading to its stabilization.
Function E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1 and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of IRF3, hence attenuating type I interferon (IFN)-dependent immune responses. Represses the innate antiviral response by facilitating the formation of the NMI-IFI35 complex through 'Lys-63'-linked ubiquitination of NMI.
Protein Name E3 Ubiquitin-Protein Ligase Trim21
52 Kda Ro Protein
52 Kda Ribonucleoprotein Autoantigen Ro/Ss-A
Ring Finger Protein 81
Ring-Type E3 Ubiquitin Transferase Trim21
Sjoegren Syndrome Type A Antigen
Tripartite Motif-Containing Protein 21
Database Links Reactome: R-HSA-1834941
Reactome: R-HSA-3134975
Reactome: R-HSA-877300
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Cytoplasmic Vesicle
Enters The Nucleus Upon Exposure To Nitric Oxide
Localizes To Small Dot- Or Rod-Like Structures In The Cytoplasm
Called Processing Bodies (P-Bodies) That Are Located Underneath The Plasma Membrane And Also Diffusely In The Cytoplasm
They Are Located Along The Microtubules And Are Highly Motile In Cells
Colocalizes With Dcp2 In P-Bodies
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Trim21 antibody
Anti-52 Kda Ro Protein antibody
Anti-52 Kda Ribonucleoprotein Autoantigen Ro/Ss-A antibody
Anti-Ring Finger Protein 81 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Trim21 antibody
Anti-Ro(Ss-A antibody
Anti-Sjoegren Syndrome Type A Antigen antibody
Anti-Ss-A antibody
Anti-Tripartite Motif-Containing Protein 21 antibody
Anti-TRIM21 antibody
Anti-RNF81 antibody
Anti-RO52 antibody
Anti-SSA1 antibody

Information sourced from Uniprot.org

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