• Chromatin immunoprecipitation analysis of extracts of 293F cells, using TriMethyl-Histone H3-K4 Rabbit polyclonal antibody antibody (STJ23983) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunohistochemistry analysis of TriMethyl-Histone H3-K4 in paraffin-embedded human colon carcinoma using TriMethyl-Histone H3-K4 Rabbit polyclonal antibody (STJ23983) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TriMethyl-Histone H3-K4 in paraffin-embedded human breast cancer using TriMethyl-Histone H3-K4 Rabbit polyclonal antibody (STJ23983) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K4 antibody (STJ23983) at 1:1000 dilution.
  • Western blot analysis of Arabidopsis thaliana (Before inflorescence) , using TriMethyl-Histone H3-K4 Rabbit polyclonal antibody (STJ23983) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates, using TriMethyl-Histone H3-K4 Rabbit polyclonal antibody (STJ23983) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Tri-Methyl-Histone H3-K4 antibody (2-19) (STJ23983)

SKU:
STJ23983

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Tri-Methyl-Histone H3-K4 (2-19) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: DB 1:500-1:2000
WB 1:100-1:500
IHC-P 1:50-1:200
ChIP 1:20-1:100
ChIP-seq 1:20-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 2-19
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 2-19 of human histone H3 (NP_003520.1).
Immunogen Sequence: ARTKQTARKSTGGKAPRK
Background The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP3) protein, CRLR functions as an adrenomedullin receptor.

Information sourced from Uniprot.org

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