• Western blot analysis of extracts of HCT116 cells, using TOPBP1 antibody (STJ28348) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using TOPBP1 rabbit polyclonal antibody (STJ28348) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse intestin using TOPBP1 rabbit polyclonal antibody (STJ28348) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using TOPBP1 rabbit polyclonal antibody (STJ28348) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-TOPBP1 antibody (1253-1522) (STJ28348)

SKU:
STJ28348

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TOPBP1 (1253-1522) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TOPBP1
Gene ID: 11073
Uniprot ID: TOPB1_HUMAN
Immunogen Region: 1253-1522
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1253-1522 of human TOPBP1 (NP_008958.2).
Immunogen Sequence: KIITIEETHEELKKQYIFQL SSLNPQERIDYCHLIEKLGG LVIEKQCFDPTCTHIVVGHP LRNEKYLASVAAGKWVLHRS YLEACRTAGHFVQEEDYEWG SSSILDVLTGINVQQRRLAL AAMRWRKKIQQRQESGIVEG AFSGWKVILHVDQSREAGFK RLLQSGGAKVLPGHSVPLFK EATHLFSDLNKLKPDDSGVN IAEAAAQNVYCLRTEYIADY LMQESPPHVENYCLPEAIS
Tissue Specificity Highly expressed in heart, brain, placenta, lung and kidney.
Post Translational Modifications Phosphorylated on serine and threonine residues in response to X-ray irradiation. Ubiquitinated and degraded by the proteasome. X-ray irradiation reduces ubiquitination. Deubiquitinated by USP13.leading to TOPBP1 stabilizion and activation of the ATR-TOPBP1 axis pathway.
Function Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR.
Protein Name Dna Topoisomerase 2-Binding Protein 1
Dna Topoisomerase Ii-Beta-Binding Protein 1
Topbp1
Dna Topoisomerase Ii-Binding Protein 1
Database Links Reactome: R-HSA-5685938
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-6804756
Reactome: R-HSA-69473
Reactome: R-HSA-9709570
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle Pole
Chromosome
Detected On Unpaired Autosomes In Meiotic Prophase Cells
Detected On X And Y Chromosomes During Later Stages Of Prophase
Colocalizes With Atr And H2ax At Unsynapsed Chromosome Cores During Prophase
Has A Uniform Nuclear Distribution During G Phase
Colocalizes With Brca1 At Stalled Replication Forks During S Phase
In Mitotic Cells It Colocalizes With Brca1 At Spindle Poles And Centrosomes During Metaphase And Anaphase
Detected In Discrete Foci Together With Pml And Numerous Dna Repair Enzymes After Dna Damage By Alkylating Agents
Uv Or Gamma Irradiation
Localizes To Sites Of Dna Damage In A H2ax- Independent Manner
Alternative Antibody Names Anti-Dna Topoisomerase 2-Binding Protein 1 antibody
Anti-Dna Topoisomerase Ii-Beta-Binding Protein 1 antibody
Anti-Topbp1 antibody
Anti-Dna Topoisomerase Ii-Binding Protein 1 antibody
Anti-TOPBP1 antibody
Anti-KIAA0259 antibody

Information sourced from Uniprot.org

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