• Immunoprecipitation analysis of 25 Mu g extracts of Mouse spleen cells using 3 Mu g TNFAIP8L2 antibody (STJ115653). Western blot was performed from the immunoprecipitate using TNFAIP8L2 antibody (STJ115653) at a dilution of 1:1000.
  • Immunofluorescence analysis of L929 cells using TNFAIP8L2 Rabbit polyclonal antibody (STJ115653) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using TNFAIP8L2 Rabbit polyclonal antibody (STJ115653) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using TNFAIP8L2 Rabbit polyclonal antibody (STJ115653) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using TNFAIP8L2 Rabbit polyclonal antibody (STJ115653) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TNFAIP8L2 antibody (1-184) (STJ115653)

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STJ115653

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TNFAIP8L2 (1-184) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TNFAIP8L2
Gene ID: 79626
Uniprot ID: TP8L2_HUMAN
Immunogen Region: 1-184
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human TNFAIP8L2 (NP_078851.2).
Immunogen Sequence: MESFSSKSLALQAEKKLLSK MAGRSVAHLFIDETSSEVLD ELYRVSKEYTHSRPQAQRVI KDLIKVAIKVAVLHRNGSFG PSELALATRFRQKLRQGAMT ALSFGEVDFTFEAAVLAGLL TECRDVLLELVEHHLTPKSH GRIRHVFDHFSDPGLLTALY GPDFTQHLGKICDGLRKLLD EGKL
Tissue Specificity Expressed in T-cells, B-cells, macrophages, neurons in the brain and brainstem, and stratified squamous epithelia of the esophagus, cervix and skin.
Post Translational Modifications Phosphorylated by TAK1/MAP3K7.this phosphorylation triggers association with BTRC and subsequent ubiquitination and degradation. Ubiquitinated in a BTRC-depdent manner.leading to degradation mediated through the proteasome pathway.
Function Acts as a negative regulator of innate and adaptive immunity by maintaining immune homeostasis. Plays a regulatory role in the Toll-like signaling pathway by determining the strength of LPS-induced signaling and gene expression. Inhibits TCR-mediated T-cell activation and negatively regulate T-cell function to prevent hyperresponsiveness. Inhibits also autolysosome formation via negatively modulating MTOR activation by interacting with RAC1 and promoting the disassociation of the RAC1-MTOR complex. Plays an essential role in NK-cell biology by acting as a checkpoint and displaying an expression pattern correlating with NK-cell maturation process and by negatively regulating NK-cell maturation and antitumor immunity. Mechanistically, suppresses IL-15-triggered mTOR activity in NK-cells.
Protein Name Tumor Necrosis Factor Alpha-Induced Protein 8-Like Protein 2
Tipe2
Tnf Alpha-Induced Protein 8-Like Protein 2
Tnfaip8-Like Protein 2
Inflammation Factor Protein 20
Database Links Reactome: R-HSA-1483255
Cellular Localisation Cytoplasm
Nucleus
Lysosome
Alternative Antibody Names Anti-Tumor Necrosis Factor Alpha-Induced Protein 8-Like Protein 2 antibody
Anti-Tipe2 antibody
Anti-Tnf Alpha-Induced Protein 8-Like Protein 2 antibody
Anti-Tnfaip8-Like Protein 2 antibody
Anti-Inflammation Factor Protein 20 antibody
Anti-TNFAIP8L2 antibody

Information sourced from Uniprot.org

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