• Western blot analysis of extracts of various cell lines, using TMEFF2 antibody (STJ112493) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TMEFF2 antibody (41-320) (STJ112493)

SKU:
STJ112493

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TMEFF2 (41-320) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TMEFF2
Gene ID: 23671
Uniprot ID: TEFF2_HUMAN
Immunogen Region: 41-320
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 41-320 of human TMEFF2 (NP_057276.2).
Immunogen Sequence: FPTSLSDCQTPTGWNCSGYD DRENDLFLCDTNTCKFDGEC LRIGDTVTCVCQFKCNNDYV PVCGSNGESYQNECYLRQAA CKQQSEILVVSEGSCATDAG SGSGDGVHEGSGETSQKETS TCDICQFGAECDEDAEDVWC VCNIDCSQTNFNPLCASDGK SYDNACQIKEASCQKQEKIE VMSLGRCQDNTTTTTKSEDG HYARTDYAENANKLEESARE HHIPCPEHYNGFCMHGKCE
Tissue Specificity Highly expressed in adult and fetal brain, spinal cord and prostate. Expressed in all brain regions except the pituitary gland, with highest levels in amygdala and corpus callosum. Expressed in the pericryptal myofibroblasts and other stromal cells of normal colonic mucosa. Expressed in prostate carcinoma. Down-regulated in colorectal cancer. Present in Alzheimer disease plaques (at protein level). Isoform 3 is expressed weakly in testis and at high levels in normal and cancerous prostate.
Post Translational Modifications O-glycosylated.contains chondroitin sulfate glycosaminoglycans. A soluble form (TMEFF2-ECD) is produced by proteolytic shedding. This shedding can be induced by phorbol ester or pro-inflammatory cytokines such as TNFalpha, and is mediated by ADAM17.
Function May be a survival factor for hippocampal and mesencephalic neurons. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation.
Protein Name Tomoregulin-2
Tr-2
Hyperplastic Polyposis Protein 1
Transmembrane Protein With Egf-Like And Two Follistatin-Like Domains
Cellular Localisation Isoform 1: Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Membrane
Isoform 3: Secreted
Alternative Antibody Names Anti-Tomoregulin-2 antibody
Anti-Tr-2 antibody
Anti-Hyperplastic Polyposis Protein 1 antibody
Anti-Transmembrane Protein With Egf-Like And Two Follistatin-Like Domains antibody
Anti-TMEFF2 antibody
Anti-HPP1 antibody
Anti-TENB2 antibody
Anti-TPEF antibody
Anti-UNQ178 antibody
Anti-PRO204 antibody

Information sourced from Uniprot.org

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