• Western blot analysis of extracts of SKOV3 cells, using TICAM1 antibody (STJ116097) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 1s.

Anti-TICAM1 antibody (150-250) (STJ116097)

SKU:
STJ116097

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TICAM1 (150-250) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TICAM1
Gene ID: 148022
Uniprot ID: TCAM1_HUMAN
Immunogen Region: 150-250
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human TICAM1 (NP_891549.1).
Immunogen Sequence: GDPGSIRTLQSNLGCLPPSS ALPSGTRSLPRPIDGVSDWS QGCSLRSTGSPASLASNLEI SQSPTMPFLSLHRSPHGPSK LCDDPQASLVPEPVPGGCQE P
Tissue Specificity Ubiquitously expressed but with higher levels in liver.
Post Translational Modifications Phosphorylated by TBK1. Following activation, phosphorylated by TBK1 at Ser-210 in the pLxIS motif. The phosphorylated pLxIS motif constitutes an IRF3-binding motif, leading to recruitment of the transcription factor IRF3 to induce type-I interferons and other cytokines. Polyubiquitinated at Lys-229 by TRIM38 with 'Lys-48'-linked chains, leading to proteasomal degradation. Polyubiquitinated with 'Lys-6'- and 'Lys-33'-linked chains in a TRIM8-dependent manner.ubiquitination disrupts the interaction with TBK1 and subsequent interferon production. (Microbial infection) Cleaved and degraded by hepatitis A virus (HAV) protein 3CD allowing the virus to disrupt host TLR3 signaling. (Microbial infection) Cleaved by CVB3 protease 3C allowing the virus to disrupt host TLR3 signaling. (Microbial infection) Cleaved by Seneca Valley virus protease 3C allowing the virus to disrupt host TLR3 signaling. (Microbial infection) Cleaved by protease 3C of human enterovirus D68 (EV68) allowing the virus to disrupt host TLR3 signaling. (Microbial infection) Cleaved by HCV protease NS3/4A, thereby disrupting TLR3 signaling and preventing the establishment of an antiviral state.
Function Involved in innate immunity against invading pathogens. Adapter used by TLR3, TLR4 (through TICAM2) and TLR5 to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively. Phosphorylation by TBK1 on the pLxIS motif leads to recruitment and subsequent activation of the transcription factor IRF3 to induce expression of type I interferon and exert a potent immunity against invading pathogens. Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines.
Protein Name Tir Domain-Containing Adapter Molecule 1
Ticam-1
Proline-Rich - Vinculin And Tir Domain-Containing Protein B
Putative Nf-Kappa-B-Activating Protein 502h
Toll-Interleukin-1 Receptor Domain-Containing Adapter Protein Inducing Interferon Beta
Myd88-3
Tir Domain-Containing Adapter Protein Inducing Ifn-Beta
Database Links Reactome: R-HSA-140534
Reactome: R-HSA-166166
Reactome: R-HSA-168164
Reactome: R-HSA-168927
Reactome: R-HSA-1810476
Reactome: R-HSA-2562578
Reactome: R-HSA-5602566
Reactome: R-HSA-5602571
Reactome: R-HSA-9013957
Reactome: R-HSA-9013973
Reactome: R-HSA-9014325
Reactome: R-HSA-936964
Reactome: R-HSA-937041
Reactome: R-HSA-937072
Reactome: R-HSA-975163
Cellular Localisation Cytoplasmic Vesicle
Autophagosome
Cytoplasm
Cytosol
Mitochondrion
Colocalizes With Ubqln1 In The Autophagosome
Colocalizes In The Cytosol With Ddx1
Ddx21 And Dhx36
Colocalizes In The Mitochondria With Ddx1 And Poly(I:c) Rna Ligand
The Multi-Helicase-Ticam1 Complex May Translocate To The Mitochondria Upon Poly(I:c) Rna Ligand Stimulation
Alternative Antibody Names Anti-Tir Domain-Containing Adapter Molecule 1 antibody
Anti-Ticam-1 antibody
Anti-Proline-Rich - Vinculin And Tir Domain-Containing Protein B antibody
Anti-Putative Nf-Kappa-B-Activating Protein 502h antibody
Anti-Toll-Interleukin-1 Receptor Domain-Containing Adapter Protein Inducing Interferon Beta antibody
Anti-Myd88-3 antibody
Anti-Tir Domain-Containing Adapter Protein Inducing Ifn-Beta antibody
Anti-TICAM1 antibody
Anti-PRVTIRB antibody
Anti-TRIF antibody

Information sourced from Uniprot.org

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