| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-THOC1 (428-657) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
THOC1 |
| Gene ID: |
9984 |
| Uniprot ID: |
THOC1_HUMAN |
| Immunogen Region: |
428-657 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 428-657 of human Nuclear Matrix Protein p84 (Nuclear Matrix Protein p84 (THOC1) ) (NP_005122.2). |
| Immunogen Sequence: |
TKKILMGNEELTRLWNLCPD NMEACKSETREHMPTLEEFF EEAIEQADPENMVENEYKAV NNSNYGWRALRLLARRSPHF FQPTNQQFKSLPEYLENMVI KLAKELPPPSEEIKTGEDED EEDNDALLKENESPDVRRDK PVTGEQIEVFANKLGEQWKI LAPYLEMKDSEIRQIECDSE DMKMRAKQLLVAWQDQEGVH ATPENLINALNKSGLSDLAE SLTNDNETNS |
| Tissue Specificity | Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression. |
| Post Translational Modifications | Expression is altered specifically during apoptosis and is accompanied by the appearance of novel forms with smaller apparent molecular mass. Polyubiquitinated, leading to proteasomal degradation.probably involves NEDD4. |
| Function | Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Regulates transcriptional elongation of a subset of genes. Involved in genome stability by preventing co-transcriptional R-loop formation. May play a role in hair cell formation, hence may be involved in hearing. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1. |
| Protein Name | Tho Complex Subunit 1Tho1Nuclear Matrix Protein P84P84n5Htrex84 |
| Database Links | Reactome: R-HSA-159236Reactome: R-HSA-72187Reactome: R-HSA-73856 |
| Cellular Localisation | Isoform 1: Nucleus SpeckleNucleusNucleoplasmNucleus MatrixCytoplasmCan Shuttle Between The Nucleus And CytoplasmNuclear Localization Is Required For Induction Of Apoptotic Cell DeathTranslocates To The Cytoplasm During The Early Phase Of Apoptosis ExecutionIsoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-Tho Complex Subunit 1 antibodyAnti-Tho1 antibodyAnti-Nuclear Matrix Protein P84 antibodyAnti-P84n5 antibodyAnti-Htrex84 antibodyAnti-THOC1 antibodyAnti-HPR1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
