• Western blot analysis of lysates from wild type (WT) and TDP2 knockout (KO) HeLa cells, using [KO Validated] TDP2 Rabbit polyclonal antibody (STJ119990) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TDP2 antibody (1-200) (STJ119990)

SKU:
STJ119990

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TDP2 (1-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TDP2
Gene ID: 51567
Uniprot ID: TYDP2_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human TDP2 (NP_057698.2).
Immunogen Sequence: MELGSCLEGGREAAEEEGEP EVKKRRLLCVEFASVASCDA AVAQCFLAENDWEMERALNS YFEPPVEESALERRPETISE PKTYVDLTNEETTDSTTSKI SPSEDTQQENGSMFSLITWN IDGLDLNNLSERARGVCSYL ALYSPDVIFLQEVIPPYYSY LKKRSSNYEIITGHEEGYFT AIMLKKSRVKLKSQEIIPFP
Tissue Specificity Widely expressed. Highly expressed in various brain regions, including the frontal and occipital lobes, the hippocampus, the striatum and the cerebellum.
Post Translational Modifications Ubiquitinated by TRAF6.
Function DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DNA double-strand breaks/DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Thereby, protects the transcription of many genes involved in neurological development and maintenance from the abortive activity of TOP2. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DSBs due to DNA damage by radiation and free radicals. Has preference for single-stranded DNA or duplex DNA with a 4 base pair overhang as substrate. Acts as a regulator of ribosome biogenesis following stress. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. Constitutes the major if not only 5'-tyrosyl-DNA phosphodiesterase in cells. Also acts as an adapter by participating in the specific activation of MAP3K7/TAK1 in response to TGF-beta: associates with components of the TGF-beta receptor-TRAF6-TAK1 signaling module and promotes their ubiquitination dependent complex formation. Involved in non-canonical TGF-beta induced signaling routes. May also act as a negative regulator of ETS1 and may inhibit NF-kappa-B activation. (Microbial infection) Also acts as a 5'-tyrosyl-RNA phosphodiesterase following picornavirus infection: its activity is hijacked by picornavirus and acts by specifically cleaving the protein-RNA covalent linkage generated during the viral genomic RNA replication steps of a picornavirus infection, without impairing the integrity of viral RNA.
Protein Name Tyrosyl-Dna Phosphodiesterase 2
Tyr-Dna Phosphodiesterase 2
Htdp2
5'-Tyrosyl-Dna Phosphodiesterase
5'-Tyr-Dna Phosphodiesterase
Ets1-Associated Protein 2
Ets1-Associated Protein Ii
Eapii
Traf And Tnf Receptor-Associated Protein
Tyrosyl-Rna Phosphodiesterase
Vpg Unlinkase
Database Links Reactome: R-HSA-5693571
Cellular Localisation Nucleus
Pml Body
Nucleolus
Cytoplasm
Localizes To Nucleolar Cavities Following Stress
Localization To Nucleolus Is Dependent On Pml Protein
(Microbial Infection) In Case Of Infection By Picornavirus
Relocalizes To Cytoplasmic Sites Distinct From Those Containing Viral Proteins Associated With Rna Replication Or Encapsidation
Alternative Antibody Names Anti-Tyrosyl-Dna Phosphodiesterase 2 antibody
Anti-Tyr-Dna Phosphodiesterase 2 antibody
Anti-Htdp2 antibody
Anti-5'-Tyrosyl-Dna Phosphodiesterase antibody
Anti-5'-Tyr-Dna Phosphodiesterase antibody
Anti-Ets1-Associated Protein 2 antibody
Anti-Ets1-Associated Protein Ii antibody
Anti-Eapii antibody
Anti-Traf And Tnf Receptor-Associated Protein antibody
Anti-Tyrosyl-Rna Phosphodiesterase antibody
Anti-Vpg Unlinkase antibody
Anti-TDP2 antibody
Anti-EAP2 antibody
Anti-TTRAP antibody
Anti-AD-022 antibody

Information sourced from Uniprot.org

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