• Western blot analysis of lysates from wild type (WT) and TAF12 knockout (KO) HeLa cells, using [KO Validated] TAF12 Rabbit polyclonal antibody (STJ11100046) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-TAF12 antibody (1-161) (STJ11100046)

SKU:
STJ11100046

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TAF12 (1-161) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TAF12
Gene ID: 6883
Uniprot ID: TAF12_HUMAN
Immunogen Region: 1-161
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human TAF12 (NP_001128690.1).
Immunogen Sequence: MNQFGPSALINLSNFSSIKP EPASTPPQGSMANSTAVVKI PGTPGAGGRLSPENNQVLTK KKLQDLVREVDPNEQLDEDV EEMLLQIADDFIESVVTAAC QLARHRKSSTLEVKDVQLHL ERQWNMWIPGFGSEEIRPYK KACTTEAHKQRMALIRKTTK K
Tissue Specificity Ubiquitous.
Function The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. Component of the TATA-binding protein-free TAF complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex.
Protein Name Transcription Initiation Factor Tfiid Subunit 12
Transcription Initiation Factor Tfiid 20/15 Kda Subunits
Tafii-20/Tafii-15
Tafii20/Tafii15
Database Links Reactome: R-HSA-167161
Reactome: R-HSA-167162
Reactome: R-HSA-167172
Reactome: R-HSA-3214847
Reactome: R-HSA-674695
Reactome: R-HSA-6804756
Reactome: R-HSA-73776
Reactome: R-HSA-73779
Reactome: R-HSA-75953
Reactome: R-HSA-76042
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Initiation Factor Tfiid Subunit 12 antibody
Anti-Transcription Initiation Factor Tfiid 20/15 Kda Subunits antibody
Anti-Tafii-20/Tafii-15 antibody
Anti-Tafii20/Tafii15 antibody
Anti-TAF12 antibody
Anti-TAF15 antibody
Anti-TAF2J antibody
Anti-TAFII20 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance