• Western blot analysis of extracts of various cell lines, using Synaptophysin antibody (STJ11101659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of Rat brain using Synaptophysin antibody (STJ11101659) at dilution of 1 : 100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse brain using Synaptophysin antibody (STJ11101659) at dilution of 1 : 100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SYP antibody (200-300) [S9MR] (STJ11101659)

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STJ11101659

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Synaptophysin (200-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SYP
Gene ID: 6855
Uniprot ID: SYPH_HUMAN
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Synaptophysin (NP_003170.1).
Immunogen Sequence: GLNTSVVFGFLNLVLWVGNL WFVFKETGWAAPFLRAPPGA PEKQPAPGDAYGDAGYGQGP GGYGPQDSYGPQGGYQPDYG QPAGSGGSGYGPQGDYGQQG Y
Tissue Specificity Expressed in the brain, with expression in the hippocampus, the neuropil in the dentate gyrus, where expression is higher in the outer half of the molecular layer than in the inner half, and in the neuropil of CA4 and CA3. Expressed in the putamen (at protein level).
Post Translational Modifications Ubiquitinated.mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. Phosphorylated by SRC.
Function Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
Protein Name Synaptophysin
Major Synaptic Vesicle Protein P38
Database Links Reactome: R-HSA-9662360
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Multi-Pass Membrane Protein
Synapse
Synaptosome
Alternative Antibody Names Anti-Synaptophysin antibody
Anti-Major Synaptic Vesicle Protein P38 antibody
Anti-SYP antibody

Information sourced from Uniprot.org

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