| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SUPT4H1 (1-117) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SUPT4H1 |
| Gene ID: |
6827 |
| Uniprot ID: |
SPT4H_HUMAN |
| Immunogen Region: |
1-117 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human SUPT4H1 (NP_003159.1). |
| Immunogen Sequence: |
MALETVPKDLRHLRACLLCS LVKTIDQFEYDGCDNCDAYL QMKGNREMVYDCTSSSFDGI IAMMSPEDSWVSKWQRVSNF KPGVYAVSVTGRLPQGIVRE LKSRGVAYKSRDTAIKT |
| Tissue Specificity | Widely expressed. |
| Function | Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. |
| Protein Name | Transcription Elongation Factor Spt4Hspt4Drb Sensitivity-Inducing Factor 14 Kda SubunitDsif P14Drb Sensitivity-Inducing Factor Small SubunitDsif Small Subunit |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-113418Reactome: R-HSA-167152Reactome: R-HSA-167158Reactome: R-HSA-167200Reactome: R-HSA-167238Reactome: R-HSA-167242Reactome: R-HSA-167243Reactome: R-HSA-167246Reactome: R-HSA-167287Reactome: R-HSA-167290Reactome: R-HSA-674695Reactome: R-HSA-6796648Reactome: R-HSA-6807505Reactome: R-HSA-75955 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Elongation Factor Spt4 antibodyAnti-Hspt4 antibodyAnti-Drb Sensitivity-Inducing Factor 14 Kda Subunit antibodyAnti-Dsif P14 antibodyAnti-Drb Sensitivity-Inducing Factor Small Subunit antibodyAnti-Dsif Small Subunit antibodyAnti-SUPT4H1 antibodyAnti-SPT4H antibodyAnti-SUPT4H antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
