• Western blot analysis of extracts of various cell lines, using STK39 rabbit monoclonal antibody (STJ11101802) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of mouse testis, using STK39 rabbit monoclonal antibody (STJ11101802) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human colon using STK39 rabbit monoclonal antibody (STJ11101802) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using STK39 rabbit monoclonal antibody (STJ11101802) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Confocal imaging of Hep G2 cells using STK39 rabbit monoclonal antibody (STJ11101802, at dilution of 1:100) (Red). The cells were counterstained with Alpha-Tubulin mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Confocal imaging of human colon using STK39 rabbit monoclonal antibody (STJ11101802, at dilution of 1:100) (Red). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol.
  • Confocal imaging of NIH/3T3 cells using STK39 rabbit monoclonal antibody (STJ11101802, at dilution of 1:100) (Red). The cells were counterstained with Alpha-Tubulin mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-STK39 antibody (401-545) [S2MR] (STJ11101802)

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STJ11101802

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-STK39 (401-545) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STK39
Gene ID: 27347
Uniprot ID: STK39_HUMAN
Immunogen Region: 401-545
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 401-545 of human STK39 (Q9UEW8).
Immunogen Sequence: SQEKSRRVKEENPEIAVSAS TIPEQIQSLSVHDSQGPPNA NEDYREASSCAVNLVLRLRN SRKELNDIRFEFTPGRDTAD GVSQELFSAGLVDGHDVVIV AANLQKIVDDPKALKTLTFK LASGCDGSEIPDEVKLIGFA QLSVS
Tissue Specificity Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis.
Post Translational Modifications Phosphorylation at Thr-231 by WNK kinases (WNK1, WNK2, WNK3 or WNK4) is required for activation. Autophosphorylation at Thr-231 positively regulates its activity. Phosphorylated at Ser-309 by PRKCQ.
Function Effector serine/threonine-protein kinase component of the WNK-SPAK/OSR1 kinase cascade, which is involved in various processes, such as ion transport, response to hypertonic stress and blood pressure. Specifically recognizes and binds proteins with a RFXV motif. Acts downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4): following activation by WNK kinases, catalyzes phosphorylation of ion cotransporters, such as SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity. Mediates regulatory volume increase in response to hyperosmotic stress by catalyzing phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1 and SLC12A6/KCC3 downstream of WNK1 and WNK3 kinases. Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx.simultaneously, phosphorylation of K-Cl cotransporters SLC12A5/KCC2 and SLC12A6/KCC3 inhibit their activity, blocking ion efflux. Acts as a regulator of NaCl reabsorption in the distal nephron by mediating phosphorylation and activation of the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney downstream of WNK4. Mediates the inhibition of SLC4A4, SLC26A6 as well as CFTR activities. Phosphorylates RELT.
Protein Name Ste20/Sps1-Related Proline-Alanine-Rich Protein Kinase
Ste-20-Related Kinase
Dcht
Serine/Threonine-Protein Kinase 39
Cellular Localisation Cytoplasm
Nucleus
Nucleus When Caspase-Cleaved
Alternative Antibody Names Anti-Ste20/Sps1-Related Proline-Alanine-Rich Protein Kinase antibody
Anti-Ste-20-Related Kinase antibody
Anti-Dcht antibody
Anti-Serine/Threonine-Protein Kinase 39 antibody
Anti-STK39 antibody
Anti-PASK antibody
Anti-SPAK antibody

Information sourced from Uniprot.org

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