Western blot analysis of extracts of various cell lines, using STK10 antibody (STJ116470) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-STK10 antibody (320-510) (STJ116470)

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STJ116470
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STK10
Gene ID: 6793
Uniprot ID: STK10_HUMAN
Immunogen Region: 320-510
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 320-510 of human STK10 (NP_005981.3).
Immunogen Sequence: DGRDEGEEEDAVDAASTLEN HTQNSSEVSPPSLNADKPLE ESPSTPLAPSQSQDSVNEPC SQPSGDRSLQTTSPPVVAPG NENGLAVPVPLRKSRPVSMD ARIQVAQEKQVAEQGGDLSP AANRSQKASQSRPNSSALET LGGEKLANGSLEPPAQAAPG PSKRDSDCSSLCTSESMDYG TNLSTDLSLNK
Tissue Specificity Highly expressed in rapidly proliferating tissues (spleen, placenta, and peripheral blood leukocytes). Also expressed in brain, heart, skeletal muscle, colon, thymus, kidney, liver, small intestine and lung.
Post Translational Modifications Autophosphorylates following homodimerization, leading to activation of the protein.
Function Serine/threonine-protein kinase involved in regulation of lymphocyte migration. Phosphorylates MSN, and possibly PLK1. Involved in regulation of lymphocyte migration by mediating phosphorylation of ERM proteins such as MSN. Acts as a negative regulator of MAP3K1/MEKK1. May also act as a cell cycle regulator by acting as a polo kinase kinase: mediates phosphorylation of PLK1 in vitro.however such data require additional evidences in vivo.
Protein Name Serine/Threonine-Protein Kinase 10
Lymphocyte-Oriented Kinase
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase 10 antibody
Anti-Lymphocyte-Oriented Kinase antibody
Anti-STK10 antibody
Anti-LOK antibody

Information sourced from Uniprot.org

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