| Host: | Rabbit |
| Applications: | WB/IHC-P/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | STIM1 |
| Gene ID: | 6786 |
| Uniprot ID: | STIM1_HUMAN |
| Immunogen Region: | 451-685 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 451-685 of human STIM1 (NP_003147.2). |
| Immunogen Sequence: | VAALNIDPSWMGSTRPNPAH FIMTDDVDDMDEEIVSPLSM QSPSLQSSVRQRLTEPQHGL GSQRDLTHSDSESSLHMSDR QRVAPKPPQMSRAADEALNA MTSNGSHRLIEGVHPGSLVE KLPDSPALAKKALLALNHGL DKAHSLMELSPSAPPGGSPH LDSSRSHSPSSPDPDTPSPV GDSRALQASRNTRIPHLAGK KAVAEEDNGSIGEETDSSPG RKKFPLKIFKKPLKK |
| Tissue Specificity | Ubiquitously expressed in various human primary cells and tumor cell lines. |
| Post Translational Modifications | Glycosylation is required for cell surface expression. Phosphorylated predominantly on Ser residues. |
| Function | Acts as a Ca(2+) sensor that gates two major inward rectifying Ca(2+) channels at the plasma membrane: Ca(2+) release-activated Ca(2+) (CRAC) channels and arachidonate-regulated Ca(2+)-selective (ARC) channels. Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates CRAC channel pore-forming subunits ORA1, ORA2 and ORAI3 to generate sustained and oscillatory Ca(2+) entry. Involved in enamel formation. |
| Protein Name | Stromal Interaction Molecule 1 |
| Database Links | Reactome: R-HSA-139853 Reactome: R-HSA-5578775 Reactome: R-HSA-983695 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Endoplasmic Reticulum Membrane Cytoplasm Cytoskeleton Sarcoplasmic Reticulum Translocates From The Endoplasmic Reticulum To The Cell Membrane In Response To A Depletion Of Intracellular Calcium And Is Detected At Punctae Corresponding To Junctions Between The Endoplasmic Reticulum And The Cell Membrane Associated With The Microtubule Network At The Growing Distal Tip Of Microtubules Colocalizes With Orai1 At The Cell Membrane Colocalizes Preferentially With Casq1 At Endoplasmic Reticulum In Response To A Depletion Of Intracellular Calcium |
| Alternative Antibody Names | Anti-Stromal Interaction Molecule 1 antibody Anti-STIM1 antibody Anti-GOK antibody |
Information sourced from Uniprot.org