| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | Dppa3 |
| Gene ID: | 73708 |
| Uniprot ID: | DPPA3_MOUSE |
| Immunogen Region: | 1-150 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human Stella (NP_631964.1). |
| Immunogen Sequence: | MEEPSEKVDPMKDPETPQKK DEEDALDDTDVLQPETLVKV MKKLTLNPGVKRSARRRSLR NRIAAVPVENKSEKIRREVQ SAFPKRRVRTLLSVLKDPIA KMRRLVRIEQRQKRLEGNEF ERDSEPFRCLCTFCHYQRWD PSENAKIGKN |
| Tissue Specificity | Expressed in the immature oocytes and in newborn ovaries. Subsequently detected in maturing oocytes and in preimplantation embryos. Expressed in pluripotent embryonic but not in differentiated somatic cells. Expressed in blastocysts, epiblasts, primordial germ cells, embryonic gonads and primitive spermatogonia. No expression is detected in adult testes. |
| Function | Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis. |
| Protein Name | Developmental Pluripotency-Associated Protein 3 Compaction-Associated Protein 1 Primordial Germ Cell Protein 7 Stella |
| Cellular Localisation | Nucleus Cytoplasm Localized In The Cytoplasm At The Primary Oocyte Stage And In Oocytes Within Mono-Laminar Follicles Expressed In The Nucleus And Cytoplasm Of Oocytes In Bi-Laminar And Graafian Follicles And During The 2-Cell And Morula Stages In 3 5 Dpc Blastocysts Localization Is Mainly Nuclear Mainly Localizes In The Female Pronucleus Localization To The Male Pronucleus In Much Weaker |
| Alternative Antibody Names | Anti-Developmental Pluripotency-Associated Protein 3 antibody Anti-Compaction-Associated Protein 1 antibody Anti-Primordial Germ Cell Protein 7 antibody Anti-Stella antibody Anti-Dppa3 antibody Anti-Cap1p antibody Anti-Crg1 antibody Anti-Pgc7 antibody |
Information sourced from Uniprot.org