| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Stella (1-150) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Dppa3 |
| Gene ID: |
73708 |
| Uniprot ID: |
DPPA3_MOUSE |
| Immunogen Region: |
1-150 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human Stella (NP_631964.1). |
| Immunogen Sequence: |
MEEPSEKVDPMKDPETPQKK DEEDALDDTDVLQPETLVKV MKKLTLNPGVKRSARRRSLR NRIAAVPVENKSEKIRREVQ SAFPKRRVRTLLSVLKDPIA KMRRLVRIEQRQKRLEGNEF ERDSEPFRCLCTFCHYQRWD PSENAKIGKN |
| Tissue Specificity | Expressed in the immature oocytes and in newborn ovaries. Subsequently detected in maturing oocytes and in preimplantation embryos. Expressed in pluripotent embryonic but not in differentiated somatic cells. Expressed in blastocysts, epiblasts, primordial germ cells, embryonic gonads and primitive spermatogonia. No expression is detected in adult testes. |
| Function | Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis. |
| Protein Name | Developmental Pluripotency-Associated Protein 3Compaction-Associated Protein 1Primordial Germ Cell Protein 7Stella |
| Cellular Localisation | NucleusCytoplasmLocalized In The Cytoplasm At The Primary Oocyte Stage And In Oocytes Within Mono-Laminar FolliclesExpressed In The Nucleus And Cytoplasm Of Oocytes In Bi-Laminar And Graafian Follicles And During The 2-Cell And Morula StagesIn 35 Dpc Blastocysts Localization Is Mainly NuclearMainly Localizes In The Female PronucleusLocalization To The Male Pronucleus In Much Weaker |
| Alternative Antibody Names | Anti-Developmental Pluripotency-Associated Protein 3 antibodyAnti-Compaction-Associated Protein 1 antibodyAnti-Primordial Germ Cell Protein 7 antibodyAnti-Stella antibodyAnti-Dppa3 antibodyAnti-Cap1p antibodyAnti-Crg1 antibodyAnti-Pgc7 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
